| Target | |
|---|---|
| Synonyms | T3E, TCRE, IMD18, CD3epsilon and T3G, IMD17, CD3GAMMA, CD3-GAMMA |
| Description | Recombinant human CD3E-knob protein with C-terminal 6×His tag and human CD3G-Hole protein with C-Strep tag |
| Delivery | In Stock |
| Uniprot ID | P07766 and P09693 |
| Expression Host | HEK293 |
| Tag | C-6×His tag and C-Strep tag |
| Molecular Characterization | knob-CD3E(Asp23-Asp126)+6×His tag Hole-CD3G(Gln23-Ser116)+Strep tag |
| Molecular Weight | The protein has a predicted molecular mass of 39.2 kDa and 40.0 kDa after removal of the signal peptide. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | T-cell surface glycoprotein CD3 delta & CD3 gamma chain, also known as CD3D & CD3G or CD3D&CD3G respectively, are single-pass type I membrane proteins. CD3D, together with CD3- epsilon(CD3E) , CD3-gamma and CD3-zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T cell receptor-CD3 complex. T cell receptor-CD3 complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. |
| Usage | Research use only |
Human CD3E-knob and CD3G-Hole Heterodimer Protein, His Tag and Twin Strep tag
Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1.Human CD3E-knob and CD3G-Hole Heterodimer Protein, His Tag and Twin Strep tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human CD3E-knob and CD3G-Hole Heterodimer Protein, His Tag and Twin Strep Tag (PME101928) can bind Anti-CD3E antibody(1G2), IgG1 Chimeric mAb (DMC101085) in a linear range of 8.0-40 ng/mL.
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