ELISA, Flow Cyt
ELISA 1/5000-10000;Flow Cyt 1/100
Purified from cell culture supernatant by affinity chromatography
|Formulation & Reconstitution||
Research use only
This gene encodes a leucine-rich transmembrane glycoprotein that may be involved in cell adhesion. The encoded protein is an oncofetal antigen that is specific to trophoblast cells. In adults this protein is highly expressed in many tumor cells and is associated with poor clinical outcome in numerous cancers. Alternate splicing in the 5' UTR results in multiple transcript variants that encode the same protein.
Anti-5T4 antibody(DM137), Rabbit mAb
Price: 10μg $99.00 ; 100 μg $438.00 ; 500 μg $1314.00
Figure 1. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human 5T4 protein, His tagged protein (PME100123) can bind Rabbit anti-5T4 monoclonal antibody(clone: DM137) in a linear range of 0.1-16 ng/ml.
Figure 2. 5T4 protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-5T4 (DM137) on Expi293 cells transfected with human 5T4 (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram),and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram)