| Clone ID | DM45 |
|---|---|
| Target | |
| Synonyms | SDC1; Syndecan-1; CD138; SYND1; SDC |
| Host Species | Rabbit |
| Description | Anti-CD138 antibody(DM45); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P18827 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA IHC FC |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Endotoxin | Less than 1.0 EU/μg by the LAL method. For <1 EU/mg requirements, please contact us for customization. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | Syndecan-1 (SYND1 or SDC1) is also known as CD antigen CD138; is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding; cell signaling; and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 : SDC1 protein functions as an integral membrane protein and participates in cell proliferation; cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a useful marker for plasma cells; but only if the cells tested are already known to be derived from blood. |
| Usage | Research use only |
| Conjugate | Unconjugated |
| DIMA Disclaimer | All DIMA recombinant antibodies are genuinely generated by DIMA Biotech. They are all under patent application. Any protein sequencing or reverse engineering attempt is prohibited. We are actively scr |
Anti-CD138 antibody(DM45); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. HEK293 cell line transfected with irrelevant protein (A) and human CD138 (B) were surface stained with Rabbit anti-CD138 monoclonal antibody 1μg/ml ( clone: DM45) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 2. Flow cytometry analysis of antigen binding of rabbit anti-human CD138 mAb (DME100044). (A) DME100044 does not bind to Jurkat cells that do not express CD138. (B) A clear peak shift of DME100044 was seen compared to the control when incubated with CD138-expressing MM.1S cells, indicating strong binding of DME100044 to CD138. Antibodies were incubated at 5 µg/mL.
Figure 3. DME100044 at 5µg/ml staining CD138 in RPMI-8226 MM xenografts in NSG mice by IHC (SKU# DME100044, DM45);
Figure 4. DME100044 at 10µg/ml staining CD138 in human tonsil tissue by IHC (SKU# DME100044).
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