ELISA, Flow Cyt
ELISA 1/5000-10000;Flow Cyt 1/100
Purified from cell culture supernatant by affinity chromatography
|Formulation & Reconstitution||
Research use only
This gene is a member of the immunoglobulin superfamily and encodes a protein which transmits an inhibitory signal to T cells. The protein contains a V domain, a transmembrane domain, and a cytoplasmic tail. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. The membrane-bound isoform functions as a homodimer interconnected by a disulfide bond, while the soluble isoform functions as a monomer. Mutations in this gene have been associated with insulin-dependent diabetes mellitus, Graves disease, Hashimoto thyroiditis, celiac disease, systemic lupus erythematosus, thyroid-associated orbitopathy, and other autoimmune diseases.
Anti-CTLA-4 antibody(DM50), Rabbit mAb
Price: 10μg $99.00 ; 100 μg $438.00 ; 500 μg $1314.00
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CTLA4 protein, mFc-His tagged protein (PME100017) can bind Rabbit anti-CTLA4 monoclonal antibody (clone: DM50) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human CTLA4 (B) were surface stained with Rabbit anti-CTLA4 monoclonal antibody 1μg/ml (clone: DM50) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti-CTLA4 monoclonal antibody (clone: DM50) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Affinity ranking of different Rabbit anti-CTLA4 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.