| Clone ID | OTIR5G2 |
|---|---|
| Target | |
| Synonyms | DDK; FLAG; DYKDDDDK |
| Host Species | Rabbit |
| Description | DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags |
| Delivery | 1-2week |
| Uniprot ID | N/A |
| IgG type | IgG |
| Clonality | Monoclonal |
| Reactivity | N/A |
| Applications | ELISA；FC；IF；IHC；IP；WB |
| Recommended Dilutions | WB 1:3000, ELISA 1:5000, IP 1:1000 |
| Purification | Purified from cell culture supernatant by affinity chromatography (protein A/G) |
| Formulation & Reconstitution | PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide. |
| Storage&Shipping | Shipped at -20°C or with ice packs, Upon delivery store at -20°C. Dilute in PBS(pH7.3) if necessary. Stable for 12 months from date of receipt. Avoid repeated freeze-thaws. |
| Background | Flag is widely used as an additional tag in expression vectors. The tag can be fused with the target protein at the N-terminal and C-terminal, and can be detected. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Anti-DDK (FLAG) Antibody (OTIR5G2)，Rabbit mAb
Price: 100ul $519.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1.Immunoblot analysis of 6 different C-terminal Myc/DDK tagged overexpression lysates(Lane 1.CST4;2.LSM1;3.ALDOB;4.ALDH3A1;5.PLK1;6.METTL16) with DME905594 at 1:3000.
Figure 2.Immunoblot analysis of picked 6 different DDK tagged overexpression lysates with DME905594 at 1:3000.(Lane 1/2 N-terminal DDK tag;Lane 3/4 C-terminal 3XDDK tag; Lane 5/6 N-terminal 3XDDK tag.)
Figure 3.Flow cytometric analysis of living 293T cells transfected with human CD37 plasmid (Red) using an anti-DDK antibody (DME905594), compared to an isotype control (green) and a PBS control (blue)(1:100).
Figure 4.Immunohistology staining on IL6 overexpressed cytosection with rabbit anti DDK clone OTIR5G2 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit. Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone, 5 micron sections, 40x magnification.
Figure 5.Immunohistology staining on CLDN22 overexpressed cytosection with rabbit anti DDK clone OTIR5G2 at 1:2000 dilution 20m RT. Antibody staining was achieved with HIER Citrate pH6 , Polink1 with DAB chromogen detection kit. Positive stain shown with the brown chromogen present. HEK293T cells were transfected with cDNA clone, 5 micron sections, 40x magnification.
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