| Clone ID | DM130 |
|---|---|
| Target | |
| Synonyms | EGFR;ERBB;ERBB1;HER1;PIG61;mENA |
| Host Species | Rabbit |
| Description | Anti-EGFR antibody(DM130); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P00533 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer. |
| Usage | Research use only |
Anti-EGFR antibody(DM130); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $438.00 ; 500 μg $1314.00
Product Data
Images
Figure 1. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human EGFR protein, His tagged protein (PME100099) can bind Rabbit anti-EGFR monoclonal antibody(clone: DM130) in a linear range of 0.06-12 ng/ml.
Figure 2. EGFR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EGFR (DM130) on Expi293 cells transfected with human EGFR(Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram),and Isotype antibody on Expi293 transfected with irrelevant protein(Orange histogram)