| Target | |
|---|---|
| Synonyms | PDCD1, PD1, CD279, SLEB2 |
| Description | Recombinant human PD-1 protein with C-terminal mouse Fc and 6×His Avi tag |
| Delivery | In STOCK |
| Uniprot ID | Q15116 |
| Expression Host | HEK293 |
| Tag | C-Mouse Fc and 6×His Avi tag |
| Molecular Characterization | PD-1(Phe24-Val170)+mFc(Pro99-Lys330)+6×His+Avi tag |
| Molecular Weight | The protein has a predicted molecular mass of 45.3 kDa after removal of the signal peptide. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Sterility | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature. |
| Background | This protein is expressed in pro-B-cells and is thought to play a role in their differentiation. In mice, expression of this gene is induced in the thymus when anti-CD3 antibodies are injected and large numbers of thymocytes undergo apoptosis. Mice deficient for this gene bred on a BALB/c background developed dilated cardiomyopathy and died from congestive heart failure. These studies suggest that this gene product may also be important in T cell function and contribute to the prevention of autoimmune diseases. |
| Usage | Research use only |
| Conjugate | Biotinylated |
Biotinylated Human PD-1 Protein, mFc-His tag
Price:10μg $96.00; 50μg $370.00 ; 100 μg $555.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Biotinylated Human PD-1 Protein, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Anti-PD-1 (pembrolizumab biosimilar) mAb (BME100006) can bind Biotinylated Human PD-1 Protein, mFc-His tag (PME100025B) in a linear range of 8–40 ng/mL. In order to specifically detect PME100025B, HRP Conjugated-Streptavidin was used as detection antibody.
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