Tag | C-Flag&Strep Tag |
---|---|
Target | |
Synonyms | ADRA2L1; ADRA2RL1; ADRARL1; alpha-2BAR; ALPHA2BAR; FAME2 |
Description | Human ADRA2B-Strep full length protein-synthetic nanodisc |
Delivery | In Stock |
Uniprot ID | P18089 |
Expression Host | HEK293 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | Neuroactive ligand-receptor interaction |
Molecular Weight | The human full length ADRA2B-Strep protein has a MW of 50.0 kDa |
Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | This protein is a member of a subfamily of G protein-coupled receptors that regulate neurotransmitter release from sympathetic nerves and from adrenergic neurons in the central nervous system. The rank order of potency for agonists of this receptor is clonidine > norepinephrine > epinephrine = oxymetazoline > dopamine > p-tyramine = phenylephrine > serotonin > p-synephrine / p-octopamine. For antagonists, the rank order is yohimbine > chlorpromazine > phentolamine > mianserine > spiperone > prazosin > alprenolol > propanolol > pindolol. |
Usage | Research use only |
Conjugate | Unconjugated |
Human ADRA2B-Strep full length protein-synthetic nanodisc
Price: 10 μg $810.00 ; 50 μg $3960.00 ; 100 μg $7200.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag ADRA2B-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with ADRA2B-Strep-nanodisc is 6.225ng/ml.
Figure 2. Human ADRA2B-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
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