Recombinant Human B7-H4 protein with C-terminal human Fc
C-Human Fc Tag
B7-H4 (Phe29-Ala258) hFc(Glu99-Ala330)
The protein has a predicted molecular mass of 51.4 kDa after removal of the signal peptide.The apparent molecular mass of B7-H4-hFc is approximately 100-130 kDa due to glycosylation.
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
|Formulation & Reconstitution||
Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
|Storage & Shipping||
Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
This gene encodes a protein belonging to the B7 costimulatory protein family. Proteins in this family are present on the surface of antigen-presenting cells and interact with ligand bound to receptors on the surface of T cells. Studies have shown that high levels of the encoded protein has been correlated with tumor progression. A pseudogene of this gene is located on chromosome 20. Multiple transcript variants encoding different isoforms have been found for this gene.
Research use only
Human B7-H4 Protein, hFc Tag
Price: 10μg $74.00 ; 50μg $296.00 ; 100 μg $444.00
Figure 1. Human B7-H4 Protein, hFc Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human B7-H4, hFc-His tagged protein (PME100053) can bind Anti-B7-H4 Neutralizing antibody (BME100078) in a linear range of 3.2-80 ng/ml.