| Tag | C-Flag&Strep Tag |
|---|---|
| Target | |
| Synonyms | C7orf1; CPE-R2; CPETR2; HRVP1; RVP1 |
| Description | Human CLDN3-Strep full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | O15551 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Cell adhesion molecules (CAMs), Leukocyte transendothelial migration, Tight junction |
| Molecular Weight | The human full length CLDN3-Strep protein has a MW of 23.3 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Sterility | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| Background | Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein is an integral membrane protein and a component of tight junction strands. It is also a low-affinity receptor for Clostridium perfringens enterotoxin, and shares aa sequence similarity with a putative apoptosis-related protein found in rat. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human CLDN3-Strep full length protein-synthetic nanodisc
Price: 10 μg $810.00 ; 50 μg $3960.00 ; 100 μg $7200.00
Product Data Negative control Dima FAQ
Images Negative control Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag CLDN3-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with CLDN3-Strep-nanodisc is 1.965ng/ml.
Figure 2. Human CLDN3-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
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