| Tag | C-Flag Tag |
|---|---|
| Target | |
| Synonyms | GNRHR1; GRHR; HH7; LHRHR; LRHR |
| Description | Human GNRHR full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | P30968 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | GnRH signaling pathway, Neuroactive ligand-receptor interaction |
| Molecular Weight | The human full length GNRHR protein has a MW of 37.7 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The receptor for type 1 gonadotropin-releasing hormone. This receptor is a member of the seven-transmembrane, G-protein coupled receptor (GPCR) family. It is expressed on the surface of pituitary gonadotrope cells as well as lymphocytes, breast, ovary, and prostate. Following binding of gonadotropin-releasing hormone, the receptor associates with G-proteins that activate a phosphatidylinositol-calcium second messenger system. Activation of the receptor ultimately causes the release of gonadotropic luteinizing hormone (LH) and follicle stimulating hormone (FSH). Defects in this gene are a cause of hypogonadotropic hypogonadism (HH). Alternative splicing results in multiple transcript variants encoding different isoforms. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human GNRHR full length protein-synthetic nanodisc
Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Elisa plates were pre-coated with Flag Tag GNRHR-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GNRHR-Nanodisc is 6.385ng/ml.
Figure 2. WB analysis of Human GNRHR-Nanodisc with anti-Flag monoclonal antibody at 1/5000 dilution, followed by Goat Anti-Rabbit IgG HRP at 1/5000 dilution
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