elisa-FLP100097 PTGER4 Fig.1 Elisa 1

Human PTGER4 full length protein-synthetic nanodisc

Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00

SKU: FLP100097 Category:
Datasheet
MSDS
SOP
Product Data
Target

Synonyms

EP4; EP4R
Description

Human PTGER4 full length protein-synthetic nanodisc
Delivery

In Stock
Uniprot ID

P35408
Expression Host

HEK293
Protein Families

Druggable Genome, GPCR, Transmembrane
Protein Pathways

Neuroactive ligand-receptor interaction
Molecular Weight

The human full length PTGER4 protein has a MW of 53.1 kDa
Formulation & Reconstitution

Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions.
Storage & Shipping

Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
Background

The protein is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). This receptor can activate T-cell factor signaling. It has been shown to mediate PGE2 induced expression of early growth response 1 (EGR1), regulate the level and stability of cyclooxygenase-2 mRNA, and lead to the phosphorylation of glycogen synthase kinase-3. Knockout studies in mice suggest that this receptor may be involved in the neonatal adaptation of circulatory system, osteoporosis, as well as initiation of skin immune responses.
Usage

Research use only
Images

elisa-FLP100097 PTGER4 Fig.1 Elisa 1
Figure1. Elisa plates were pre-coated with Flag Tag PTGER4-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with PTGER4-Nanodisc is 3.882ng/ml.
page-FLP100097 PTGER4 Fig.2 SDS PAGE 1
Figure2. Human PTGER4-Nanodisc, Flag Tag on SDS-PAGE