elisa-FLP100021 TM4SF1 Fig.1 Elisa 1

Human TM4SF1 full length protein-MNP

Price: 50 μg $1200.00 ; 100 μg $1600.00

SKU: FLP100021 Category:
Product Data
Synonyms

M3S1, TAAL6
Uniprot ID

P30408
Expression Host

HEK293
Description

Human TM4SF1 full length protein membrane nanoparticles (MNPs)
Molecular Weight

696 bp
Delivery

3~4 weeks
Formulation & Reconstitution

Supplied in 1xPBS (pH 7.4)
Package

50 μg $1200.00 ; 100 μg $1600.00
Storage & Shipping

The product should be stored at -80°C. Please do not repeated freeze-thaw cycles.
Background

The protein is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface antigen and is highly expressed in different carcinomas.
Images

elisa-FLP100021 TM4SF1 Fig.1 Elisa 1
Figure1. Elisa plates were pre-coated with 0.5μg/per well purified human TM4SF1 full length membrane nanoparticles. Serial diluted anti-TM4SF1 monoclonal antibody (BME100159) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-TM4SF1 monoclonal antibody binding with TM4SF1 full length membrane nanoparticles is 4.174ng/ml.
fc-FLP100021 TM4SF1 Fig.2A FC 1fc-FLP100021 TM4SF1 Fig.2B FC 1fc-FLP100021 TM4SF1 Fig.2C FC 1fc-FLP100021 TM4SF1 Fig.2D FC 1
Figure2. FACS analysis of TM4SF1 MNPs
A. Negative Control 1: TM4SF1 full length membrane nanoparticles samples were stained only with Goat anti-human lgG 488 secondary antibody.
B. Negative Control 2: Control membrane nanoparticles samples were stained with anti-TM4SF1 antibody (BME100159) at 2μg/mL, followed by Goat anti-human IgG 488 secondary antibody.
C. Negative Control 3: TM4SF1 full length membrane nanoparticles samples were stained with anti-CCR8 antibody (an irrelevant antibody) at 2μg/mL, followed by Goat anti-human IgG 488 secondary antibody.
D. TM4SF1 full length membrane nanoparticles samples were stained with anti-TM4SF1 antibody (BME100159) at 2μg/mL, followed by Goat anti-human IgG 488 secondary antibody.