| Tag | C-Flag&Strep Tag |
|---|---|
| Target | |
| Synonyms | bA86F4.3; GPCR |
| Description | Human GPRC6A-Strep full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | Q5T6X5 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | N/A |
| Molecular Weight | The human full length GPRC6A-Strep protein has a MW of 104.8 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | Members of family C of the G protein-coupled receptor (GPCR) superfamily, such as GPRC6A, are characterized by an evolutionarily conserved amino acid-sensing motif linked to an intramembranous 7-transmembrane loop region. Several members of GPCR family C, including GPRC6A, also have a long N-terminal domain. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human GPRC6A-Strep full length protein-synthetic nanodisc
Price: 10 μg $810.00 ; 50 μg $3960.00 ; 100 μg $7200.00
Product Data Negative control Dima FAQ
Images Negative control Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag GPRC6A-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GPRC6A-Strep-nanodisc is 5.186ng/ml.
Figure 2. Human GPRC6A-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
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