B7-H4/VTCN1 Recombinant Proteins, Antibodies and Stable Cell Lines

B7-H4, also known as VTCN1, B7x, or B7S1, is a type I transmembrane member of the B7 family of immune-regulatory molecules. Protein expression is relatively limited in many normal tissues but can be markedly elevated in a variety of solid tumors, including gynecologic and breast malignancies. B7-H4 has been associated with an immunosuppressive tumor microenvironment and can negatively regulate T-cell proliferation, cytokine production, and effector activity. Its combination of tumor-associated cell-surface expression and immune-regulatory biology has made B7-H4 an attractive target for monoclonal antibodies, ADCs, and multispecific therapeutic formats.

DIMA BIOTECH provides human and cynomolgus B7-H4 recombinant ECD proteins, including constructs covering different extracellular regions, for ELISA, antibody screening, epitope mapping, binding characterization, and cross-species evaluation. Flow cytometry-validated monoclonal and reference antibodies, together with PE- and biotin-conjugated formats, support cell-surface B7-H4 detection and candidate benchmarking. A B7-H4-overexpressing K562 stable cell line can be used for FACS, cell-binding assays, and cell-based candidate screening, with downstream internalization and functional assays available for further evaluation.