CD3E/CD3ε Recombinant Proteins, Flow Antibodies and Reference Antibodies

CD3E encodes CD3ε, an essential signaling subunit of the T-cell receptor-CD3 complex. The TCR recognizes peptide-MHC complexes, while CD3γε, CD3δε, and CD3ζζ signaling modules convert antigen recognition into intracellular activation through ITAM motifs. CD3ε is broadly expressed on mature T cells and is therefore one of the most widely used targets for T-cell detection, activation, and redirection. CD3-binding domains are central components of T-cell engagers and multispecific antibodies that bring T cells into proximity with target cells independently of conventional peptide-MHC recognition. Because the intensity of CD3 signaling strongly influences cytotoxicity and cytokine release, affinity, epitope, valency, and molecular geometry are critical design parameters.

DIMA BIOTECH provides human and cynomolgus CD3E recombinant ECD proteins in His- and Fc-tagged formats for ELISA, SPR/BLI, antibody screening, affinity characterization, and cross-species evaluation. Flow cytometry-validated monoclonal and reference antibodies, including PE- and biotin-conjugated formats, support T-cell surface detection, CD3-arm benchmarking, receptor-occupancy studies, and assay development. These reagents are particularly suitable for early characterization of CD3 binding arms used in T-cell-engaging and multispecific antibody programs.