Anti-Tag Monoclonal Antibody

What is Anti-tag Monoclonal Antibody?

Anti-tag Monoclonal Antibody is a highly specific antibody designed to recognize and bind to a short peptide sequence (tag) introduced into recombinant proteins during expression. Common tags include His-tag, FLAG-tag, HA-tag, and myc-tag, among others. These antibodies enable the detection, purification, and characterization of recombinant proteins, facilitating their study in various applications.

What is the Anti-tag Monoclonal Antibodies’ application?

One of the primary applications of anti-tag monoclonal antibodies is in protein purification. By fusing a tag to a recombinant protein, researchers can easily purify the target protein using affinity chromatography, where the antibody binds to the tag and isolates the protein. This technique is essential for the production of high-purity proteins for structural analysis, drug screening, and therapeutic development.

Anti-tag antibodies are also widely used in Western blotting, ELISA, and flow cytometry for the detection and quantification of tagged proteins. These assays allow scientists to monitor protein expression levels, analyze protein localization, and confirm successful protein tagging in various experimental systems.

In addition, anti-tag monoclonal antibodies are valuable tools in cell biology and molecular research. For example, they can be used to track the expression of recombinant proteins in live cells or tissues, enabling real-time monitoring of protein dynamics. They are also employed in protein-protein interaction studies to detect the interaction between tagged proteins and their binding partners.

DIMA BIOTECH has developed various anti-tag antibodies covering Myc, His, Flag, EGFP, etc. Useing of them simplifies experimental workflows, improves reproducibility, and enhances the precision of protein research. Their versatility in different research fields makes them indispensable for scientists working with recombinant proteins and cell-based assays.