| Tag | C-Flag&Avi Tag |
|---|---|
| Target | |
| Synonyms | CC-CKR-2; CCR-2; CCR2A; CCR2B; CD192; CKR2; CKR2A; CKR2B; CMKBR2; MCP-1-R |
| Description | Biotinylated Human CCR2 full length protein-PeptiNanodisc |
| Delivery | In Stock |
| Uniprot ID | P41597 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Chemokine signaling pathway, Cytokine-cytokine receptor interaction |
| Molecular Weight | The human full length CCR2 protein has a MW of 41.9 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein is a receptor for monocyte chemoattractant protein-1, a chemokine which specifically mediates monocyte chemotaxis. Monocyte chemoattractant protein-1 is involved in monocyte infiltration in inflammatory diseases such as rheumatoid arthritis as well as in the inflammatory response against tumors. The encoded protein mediates agonist-dependent calcium mobilization and inhibition of adenylyl cyclase. This protein can also be a coreceptor with CD4 for HIV-1 infection. This gene is located in the chemokine receptor gene cluster region of chromosome 3. |
| Usage | Research use only |
| Conjugate | Biotinylated |
Biotinylated Human CCR2 full length protein-PeptiNanodisc
Price: 10 μg $1089.00 ; 50 μg $5324.00 ; 100 μg $9680.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Elisa plates were added with C-Flag&Avi Tag Biotinylated CCR2 peptiNanodisc (0.2μg/per well) on a Streptavidin pre-coated (0.2μg/per well) plate. Serial diluted anti-CCR2 monoclonal antibody (DMC100447) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CCR2 monoclonal antibody binding with Biotinylated CCR2-PeptiNanodisc is 155.7ng/ml.
Figure 2. Biotinylated Human CCR2-PeptiNanodisc, C-Flag&Avi Tag on SDS-PAGE
Figure 3. WB analysis of Biotinylated Human CCR2-PeptiNanodisc with anti-CCR2 monoclonal antibody (DMC100447) at 1μg/ml, followed by Goat Anti-Human IgG HRP at 1/5000 dilution
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