| Tag | C-Flag&Avi Tag |
|---|---|
| Target | |
| Synonyms | FEX; GPR49; GPR67; GRP49; HG38 |
| Description | Biotinylated Human LGR5 full length protein-PeptiNanodisc |
| Delivery | In Stock |
| Uniprot ID | O75473 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | N/A |
| Molecular Weight | The human full length LGR5 protein has a MW of 100.0 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. |
| Usage | Research use only |
| Conjugate | Biotinylated |
Biotinylated Human LGR5 full length protein-PeptiNanodisc
Price: 10 μg $1089.00 ; 50 μg $5324.00 ; 100 μg $9680.00
Product Data Dima FAQ
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Figure 1. Elisa plates were added with C-Flag&Avi Tag Biotinylated LGR5 peptiNanodisc (0.2μg/per well) on a Streptavidin pre-coated (0.2μg/per well) plate. Serial diluted anti-LGR5 monoclonal antibody (BME100297) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-LGR5 monoclonal antibody binding with Biotinylated LGR5-PeptiNanodisc is 7.709ng/ml.
Figure 2. Biotinylated Human LGR5-PeptiNanodisc, C-Flag&Avi Tag on SDS-PAGE
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