Tag | C-Flag Tag |
---|---|
Target | |
Synonyms | FEX; GPR49; GPR67; GRP49; HG38 |
Description | Human LGR5 full length protein-synthetic nanodisc |
Delivery | In Stock |
Uniprot ID | O75473 |
Expression Host | HEK293 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | N/A |
Molecular Weight | The human full length LGR5 protein has a MW of 100.0 kDa |
Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lower than 6.5 in subsequent experiments. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | The protein is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. |
Usage | Research use only |
Human LGR5 full length protein-synthetic nanodisc
Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00
Product Data
Images
Figure1. Elisa plates were pre-coated with Flag Tag LGR5-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with LGR5-Nanodisc is 5.199ng/ml.
Figure2. Human LGR5-Nanodisc, Flag Tag on SDS-PAGE
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