| Tag | N-His, C-Single Strep Tag |
|---|---|
| Expression Host | E.coli |
| Target | |
| Description | Human C5AR1 cell-free full length protein-Nanodisc |
| Synonyms | C5A; C5AR; C5R1; CD88 |
| Uniprot ID | P21730 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Complement and coagulation cascades, Neuroactive ligand-receptor interaction |
| Molecular Weight | The human C5AR1 cell-free full length protein-Nanodisc has a MW of 41.6kDa |
| Delivery | 1 week |
| Formulation & Reconstitution | Liquid, 50mM HEPES, 150mM NaCl, pH7.5 |
| Sterility | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| Storage&Shipping | Store at -80℃, Ship on dry ice. |
| Purity | >85% |
| Background | Receptor for the chemotactic and inflammatory peptide anaphylatoxin C5a. The ligand interacts with at least two sites on the receptor: a high-affinity site on the extracellular N-terminus, and a second site in the transmembrane region which activates downstream signaling events. Receptor activation stimulates chemotaxis, granule enzyme release, intracellular calcium release and superoxide anion production |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human C5AR1 cell-free full length protein-Nanodisc
Price: 20μg $1,828.00; 100μg $3,627.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Elisa plates were pre-coated with N-His, C-Single Strep Tag C5AR1 cell-free-Nanodisc (0.5μg/per well). Serial diluted anti-C5AR1 antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-C5AR1 antibody binding with C5AR1 cell-free-Nanodisc is 11.6 ng/mL.
Figure 2. Human C5AR1 cell-free-Nanodisc,N-His, C-Single Strep Tag on SDS-PAGE.
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