| Target | |
|---|---|
| Synonyms | 4-1BB Ligand,TNFSF9,CD137L |
| Description | Recombinant human 4-1BB Ligand Protein with N-terminal mouse Fc and C-terminal 6×His Avi tag |
| Delivery | In STOCK |
| Uniprot ID | P41273 |
| Expression Host | HEK293 |
| Tag | N-Mouse Fc and C-6×His Avi tag |
| Molecular Characterization | mFc(Pro99-Lys330)+4-1BB Ligand(Pro52-Glu254)+6×His+Avi tag |
| Molecular Weight | The protein has a predicted molecular mass of 51.6 kDa after removal of the signal peptide. |
| Purity | The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining. |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. |
| Sterility | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing).Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9/4-1BB, which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9/4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene, TNFSF9/4-1BBL, has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines, and is thought to be involved in T cell-tumor cell interaction. |
| Usage | Research use only |
| Conjugate | Biotinylated |
Biotinylated Human 4-1BB Ligand Protein, mFc-His Tag
Price:10μg $85.00; 50μg $330.00 ; 100 μg $500.00
Product Data Dima FAQ
Images Dima FAQ
Figure 1. Biotinylated Human 4-1BB Ligand Protein, mFc-His Tag on SDS-PAGE under reducing condition.
Figure 2. ELISA plate pre-coated by 2 μg/mL (100 μL/well) Anti-4-1BB Ligand(SC113.153 biosimilar) mAb (BME100300) can bind Biotinylated Human 4-1BB Ligand Protein, mFc-His Tag (PME100054B) in a linear range of 40–200 ng/mL. In order to specifically detect PME100054B, HRP Conjugated-Streptavidin was used as detection antibody.
Related Products
ECD Proteins
SKU: PME100054 Target: 4-1BB Ligand Tag: N-Mouse Fc and C-6×His Tag
Expression Host: HEK293
Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00
ECD Proteins
SKU: PME-C100099 Target: 4-1BB Ligand Tag: N-Human Fc tag
Expression Host: HEK293
Price:10μg $85.00; 50μg $330.00 ; 100 μg $500.00
Monoclonal antibodies
SKU: DME100068B Target: 4-1BB Ligand
Application: ELISA; Flow Cyt
Price: 10μg $139.00 ; 100 μg $670.00 ; 500 μg $1999.00
Monoclonal antibodies
SKU: DME100068 Target: 4-1BB Ligand
Application: ELISA; Flow Cyt
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
ECD Proteins
SKU: PME-M100071 Target: 4-1BB Ligand Tag: N-Human Fc Tag
Expression Host: HEK293
Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00