| Tag | C-Flag Tag |
|---|---|
| Target | |
| Synonyms | IAP; MER6; OA3 |
| Description | Human CD47 full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | Q08722 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | ECM-receptor interaction |
| Molecular Weight | The human full length CD47 protein has a MW of 35.2 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C(Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | A membrane protein involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. The encoded protein is also a receptor for the C-terminal cell binding domain of thrombospondin, and it may play a role in membrane transport and signal transduction. This gene has broad tissue distribution, and is reduced in expression on Rh erythrocytes. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human CD47 full length protein-synthetic nanodisc
Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00
Product Data Negative control Dima FAQ
Images Negative control Dima FAQ
Figure 1. Elisa plates were pre-coated with Flag Tag CD47-Nanodisc (0.2μg/per well). Serial diluted anti-CD47 monoclonal antibody (BME100050) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CD47 monoclonal antibody binding with CD47-Nanodisc is 2.959ng/ml.
Figure 2. Human CD47-Nanodisc, Flag Tag on SDS-PAGE
Figure 3. Loaded Human CD47 full length protein-synthetic nanodisc (FLP100039) on Carboxylation Biosensor, can bind Anti-CD47 Magrolimab mAb (BME100050) with an affinity constant of 0.17nM as determined in SPR assay.
Related Products
ECD Proteins
SKU: PME100008 Target: CD47
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
Biosimilar reference antibodies
SKU: BME100001 Target: CD47
Application: ELISA; Flow Cyt
Price: 50μg $82.00 ; 100 μg $162.00
DiSliceX
SKU: SLI100001 Target: BCMA, BTN3A1, CD138, CD147, CD30, CD33, CD38, CD47, CD63, CD99, CS1, GITR, GPC1, GPRC5D, ICAM-1, MUC1, PDL-1, SELPLG, TFRC, TIM3
Application: IHC
Price: 2 slides $129.00
Biosimilar reference antibodies
SKU: BME100076B Target: CD47
Application: ELISA; Flow Cyt
Price: 50μg $99.00 ; 100 μg $199.00
Overexpression Stable Cell Line
SKU: CEL100040 Target: CD47
Application: FACS Data
Price:Inquiry