| Target | |
|---|---|
| Synonyms | 4-1BB Ligand;TNFSF9;CD137L |
| Host Species | Rabbit |
| Description | Anti-4-1BB Ligand antibody(DM68); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | P41273 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This transmembrane cytokine is a bidirectional signal transducer that acts as a ligand for TNFRSF9:4-1BB; which is a costimulatory receptor molecule in T lymphocytes. This cytokine and its receptor are involved in the antigen presentation process and in the generation of cytotoxic T cells. The receptor TNFRSF9:4-1BB is absent from resting T lymphocytes but rapidly expressed upon antigenic stimulation. The ligand encoded by this gene; TNFSF9:4-1BBL; has been shown to reactivate anergic T lymphocytes in addition to promoting T lymphocyte proliferation. This cytokine has also been shown to be required for the optimal CD8 responses in CD8 T cells. This cytokine is expressed in carcinoma cell lines; and is thought to be involved in T cell-tumor cell interaction. |
| Usage | Research use only |
Anti-4-1BB Ligand antibody(DM68); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $438.00 ; 500 μg $1314.00
Product Data
Images
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human 4-1BB Ligand protein, mFc-His tagged protein (PME100054) can bind Rabbit anti-4-1BB Ligand monoclonal antibody (clone: DM68) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human 4-1BB Ligand (B) were surface stained with Rabbit anti-4-1BB Ligand monoclonal antibody 1μg/ml (clone: DM68) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
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Figure 3. Flow cytometry data of serially titrated Rabbit anti-4-1BB Ligand monoclonal antibody (clone: DM68) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.