ELISA, Flow Cyt
ELISA 1/5000-10000;Flow Cyt 1/100
Purified from cell culture supernatant by affinity chromatography
|Formulation & Reconstitution||
Research use only
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
Anti-EGFR antibody(DM129), Rabbit mAb
Price: 10μg $99.00 ; 100 μg $438.00 ; 500 μg $1314.00
Figure 1. ELISA plate pre-coated by 1 μg/ml (100 μl/well) Human EGFR protein, His tagged protein (PME100099) can bind Rabbit anti-EGFR monoclonal antibody(clone: DM129) in a linear range of 0. 1-18 ng/ml.
Figure 2. EGFR protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-EGFR (DM129) on Expi293 cells transfected with human EGFR (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram),and Isotype antibody on Expi293 transfected with irrelevant protein(Orange histogram)