| Target | |
|---|---|
| Synonyms | GPRC5D |
| Host Species | Rabbit |
| Description | Anti-GPRC5D antibody(DM61); Rabbit mAb |
| Delivery | In Stock |
| Uniprot ID | Q9NZD1 |
| IgG type | Rabbit IgG |
| Clonality | Monoclonal |
| Reactivity | Human |
| Applications | ELISA; Flow Cyt; IHC |
| Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100, IHC 1:200 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
| Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a member of the G protein-coupled receptor family; however; the specific function of this gene has not yet been determined. |
| Usage | Research use only |
Anti-GPRC5D antibody(DM61); Rabbit mAb
Price: 10μg $99.00 ; 100 μg $438.00 ; 500 μg $1314.00
Product Data
Images
Figure 1. ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human GPRC5D protein, hFc-His tagged protein (PME100066) can bind Rabbit anti-GPRC5D monoclonal antibody (clone: DM61) in a linear range of 1-100 ng/ml.
Figure 2. Expi 293 cell line transfected with irrelevant protein (A) and human GPRC5D (B) were surface stained with Rabbit anti-GPRC5D monoclonal antibody 15μg/ml (clone: DM61) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Figure 3. Flow cytometry data of serially titrated Rabbit anti- GPRC5D monoclonal antibody (clone: DM61) on H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 4. Affinity ranking of different Rabbit anti- GPRC5D mAb clones by titration of different concentration onto H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Figure 5. IHC staining of H929 cells A. with rabbit anti-GPRC5D mAb (Cat# DME100061) at 5.9 ug/ml. B. with another anti-GPRC5D mAb (Cat# DME100090) at 8.8 ug/ml. DME100061 can specifically react with GPRC5D in IHC application.