Clone ID | DM77 |
---|---|
Target | |
Synonyms | CD33;SIGLEC3;gp67 |
Host Species | Rabbit |
Description | Biotinylated Anti-CD33 antibody(DM77); Rabbit mAb |
Delivery | 2-3 weeks |
Uniprot ID | P20138 |
IgG type | Rabbit IgG |
Clonality | Monoclonal |
Reactivity | Human |
Applications | ELISA; Flow Cyt |
Recommended Dilutions | ELISA 1:5000-10000; Flow Cyt 1:100 |
Purification | Purified from cell culture supernatant by affinity chromatography |
Formulation & Reconstitution | Lyophilized from sterile PBS, pH 7.4. Normally 5 % – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state. Preferentially recognizes and binds alpha-2,3- and more avidly alpha-2,6-linked sialic acid-bearing glycans. Upon engagement of ligands such as C1q or syalylated glycoproteins; two immunoreceptor tyrosine-based inhibitory motifs (ITIMs) located in CD33 cytoplasmic tail are phosphorylated by Src-like kinases such as LCK. These phosphorylations provide docking sites for the recruitment and activation of protein-tyrosine phosphatases PTPN6:SHP-1 and PTPN11:SHP-2. In turn; these phosphatases regulate downstream pathways through dephosphorylation of signaling molecules. One of the repressive effect of CD33 on monocyte activation requires phosphoinositide 3-kinase:PI3K. |
Usage | Research use only |
Biotinylated Anti-CD33 antibody(DM77); Rabbit mAb
Price: 10μg $139.00 ; 100 μg $670.00 ; 500 μg $1999.00
Product Data
Images
Related products
Biosimilar reference antibodies
SKU: BME100015 Target: CD33
Application: Flow Cyt
Price: 50μg $82.00 ; 100 μg $162.00
Biosimilar reference antibodies
SKU: BME100015B Target: CD33
Application: Flow Cyt
Price: 50μg $99.00 ; 100 μg $199.00
DiSliceX
SKU: SLI100011 Target: B7H5, BST1, CCR2, CD33, CD70, CD93, CHI3L1, TREM2
Application: IHC
Price: 2 slides $129.00
DiSliceX
SKU: SLI100001 Target: BCMA, BTN3A1, CD138, CD147, CD30, CD33, CD38, CD47, CD63, CD99, CS1, GITR, GPC1, GPRC5D, ICAM-1, MUC1, PDL-1, SELPLG, TFRC, TIM3
Application: IHC
Price: 2 slides $129.00
DimAb monoclonal antibodies
SKU: DME100077 Target: CD33
Application: ELISA; Flow Cyt
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00