Tag | C-Flag Tag |
---|---|
Target | |
Synonyms | GPRchr2; WI31133 |
Description | Human β2AR-GPR75-BRIL full length protein-synthetic nanodisc |
Delivery | In Stock |
Uniprot ID | O95800 |
Expression Host | HEK293 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | N/A |
Molecular Weight | The human full length β2AR-GPR75-BRIL protein has a MW of 59.4 kDa |
Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Lyophilized from PBS. Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with pH lower than 6.5 in subsequent experiments. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | GPR75 is a member of the G protein-coupled receptor family and is a novel target for the clinical treatment of obesity. Human GPR75 haploinsufficiency exhibits a striking phenotype of low body fat, and GPR75 knockout mice are hypophagic and thin, improving glucose tolerance and insulin sensitivity. To enhance the expression level of GPR75, twenty-four amino acids from the β2AR receptor (MGQPGNGSAFLLAPNRSHAPDHDV) were used to replace the original residues1-31 in a modified version of the GPR75 receptor. Furthermore, the BRIL sequence was used to replace the original ICL3 loop (237-306) to enhance receptor stability. BRIL is thermostabilized apocytochrome b562 with mutations M7W, H102I, R106L, PDB ID 1M6T. |
Usage | Research use only |
Conjugate | Unconjugated |
Human β2AR-GPR75-BRIL full length protein-synthetic nanodisc
Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00
Product Data
Images
Figure 1. Elisa plates were pre-coated with Flag Tag β2AR-GPR75-BRIL-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with β2AR-GPR75-BRIL-Nanodisc is 4.678ng/ml.
Figure 2. Human β2AR-GPR75-BRIL-Nanodisc, Flag Tag on SDS-PAGE
Related Products
Monoclonal antibodies
SKU: DME101136B Target: GPR75
Application: Flow Cyt
Price: 10μg $139.00 ; 100 μg $670.00 ; 500 μg $1999.00
Monoclonal antibodies
SKU: DMC100492 Target: GPR75
Application: Flow Cyt
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Full Length Transmembrane Proteins
SKU: FLP110031 Target: GPR75
Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00
ECD Proteins
SKU: PME100704 Target: GPR75
Price: 10μg $68.00; 50μg $259.00 ; 100 μg $389.00
Overexpression Stable Cell Line
SKU: CEL100092 Target: GPR75
Application: FACS Data
Price:Inquiry