Tag | C-Flag&Strep Tag |
---|---|
Target | |
Synonyms | GPRchr2; WI31133 |
Description | Human GPR75-Strep full length protein-synthetic nanodisc |
Delivery | 6~8weeks |
Uniprot ID | O95800 |
Expression Host | HEK293 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
Protein Pathways | N/A |
Molecular Weight | The human full length GPR75-Strep protein has a MW of 59.4 kDa |
Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | G protein-coupled receptor that is activated by the chemokine CCL5/RANTES. Probably coupled to heterotrimeric Gq proteins, it stimulates inositol trisphosphate production and calcium mobilization upon activation. Together with CCL5/RANTES, may play a role in neuron survival through activation of a downstream signaling pathway involving the PI3, Akt and MAP kinases. CCL5/RANTES may also regulate insulin secretion by pancreatic islet cells through activation of this receptor. |
Usage | Research use only |
Conjugate | Unconjugated |
Human GPR75-Strep full length protein-synthetic nanodisc
Price: 10 μg $810.00 ; 50 μg $3960.00 ; 100 μg $7200.00
Product Data
Images
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag GPR75-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-GPR75 monoclonal antibody (DMC100368) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-GPR75 monoclonal antibody binding with GPR75-Strep-Nanodisc is 15.14ng/ml.
Figure 2. Human GPR75-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
Figure 3. WB analysis of GPR75-Strep-Nanodisc with anti-GPR75 monoclonal antibody (DMC100368), followed by Goat Anti-Human IgG HRP at 1/5000 dilution
Related Products
Monoclonal antibodies
SKU: DMC100368B Target: GPR75
Application: Flow Cyt
Price: 10μg $139.00 ; 100 μg $670.00 ; 500 μg $1999.00
Full Length Transmembrane Proteins
Human β2AR-GPR75-BRIL full length protein-synthetic nanodisc
SKU: FLP100031A Target: GPR75
Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00
Monoclonal antibodies
SKU: DME101136 Target: GPR75
Application: Flow Cyt
Price: 10μg $99.00 ; 100 μg $446.00 ; 500 μg $1340.00
Monoclonal antibodies
PE-conjugated Anti-GPR75 antibody(DMC492); IgG1 Chimeric mAb
SKU: DMC100492P Target: GPR75
Application: Flow Cyt
Price: 100 test $550.00
Monoclonal antibodies
PE-conjugated Anti-GPR75 antibody(DMC368); IgG1 Chimeric mAb
SKU: DMC100368P Target: GPR75
Application: Flow Cyt
Price: 100 test $550.00