| Tag | C-Flag&Strep Tag |
|---|---|
| Expression Host | HEK293 |
| Target | |
| Synonyms | GPRC1E; mGlu5; MGLUR5; PPP1R86 |
| Description | Human GRM5-Strep full length protein-synthetic nanodisc |
| Uniprot ID | P41594 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Calcium signaling pathway, Gap junction, Huntington's disease, Long-term depression, Long-term potentiation, Neuroactive ligand-receptor interaction |
| Molecular Weight | The human full length GRM5-Strep protein has a MW of 132.5 kDa |
| Delivery | In Stock |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Sterility | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | A member of the G-protein coupled receptor 3 protein family. The protein is a metabatropic glutamate receptor, whose signaling activates a phosphatidylinositol-calcium second messenger system. This protein may be involved in the regulation of neural network activity and synaptic plasticity. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. A pseudogene of this gene has been defined on chromosome 11. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human GRM5-Strep full length protein-synthetic nanodisc
Price: 10 μg $810.00 ; 50 μg $3960.00 ; 100 μg $7200.00
Product Data Negative control Dima FAQ
Images Negative control Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag GRM5-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with GRM5-Strep-nanodisc is 5.898ng/ml.
Figure 2. Human GRM5-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
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