| Tag | C-Flag Tag |
|---|---|
| Expression Host | HEK293 |
| Target | |
| Synonyms | GPRC5D |
| Description | Human GPRC5D full length protein-synthetic nanodisc |
| Uniprot ID | Q9NZD1 |
| Protein Families | GPCR |
| Protein Pathways | N/A |
| Molecular Weight | The human full length GPRC5D Protein has a MW of 38.6 kDa |
| Delivery | In Stock |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Sterility | Products are supplied non-sterile. For cell culture applications, dilute in appropriate medium and sterile-filter (0.22 µm) prior to use. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein encoded by this gene is a member of the G protein-coupled receptor family. Recent studies demonstrate that GPRC5D is expressed on malignant bone marrow plasma cells, whereas normal tissue expression is limited to the hair follicle. It may represent a potential target for effector-cell–mediated therapy to treat plasma-cell disorders like MM. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human GPRC5D full length protein-synthetic nanodisc
Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00
Product Data Negative control Dima FAQ
Images Negative control Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag Tag GPRC5D-Nanodisc (0.2μg/per well). Serial diluted anti-GPRC5D monoclonal antibody (DME100091) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-GPRC5D monoclonal antibody binding with GPRC5D-Nanodisc is 20.54ng/ml.
Figure 2. Human GPRC5D-Nanodisc, Flag Tag on SDS-PAGE
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