Tag | C-Flag Tag |
---|---|
Target | |
Synonyms | GP52-40; TSPAN26 |
Description | Human CD37 full length protein-synthetic nanodisc |
Delivery | In Stock |
Uniprot ID | P11049 |
Expression Host | HEK293 |
Protein Families | Transmembrane |
Protein Pathways | Hematopoietic cell lineage |
Molecular Weight | The human full length CD37 protein has a MW of 31.7 kDa |
Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
Storage & Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
Background | The protein is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins and other transmembrane 4 superfamily proteins. It may play a role in T-cell-B-cell interactions. Alternate splicing results in multiple transcript variants encoding different isoforms. |
Usage | Research use only |
Conjugate | Unconjugated |
Human CD37 full length protein-synthetic nanodisc
Price: 10 μg $900.00 ; 50 μg $4400.00 ; 100 μg $8000.00
Product Data
Images
Figure1. Elisa plates were pre-coated with Flag Tag CD37-Nanodisc (0.2μg/per well). Serial diluted anti-CD37 monoclonal antibody (BME100046) solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-CD37 monoclonal antibody binding with CD37-Nanodisc is 23.06ng/ml.
Figure2. Human CD37-Nanodisc, Flag Tag on SDS-PAGE
Figure3. WB analysis of Human CD37-Nanodisc with anti-CD37 monoclonal antibody (BME100046), followed by Goat Anti-Human IgG HRP at 1/5000 dilution
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