Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme c100123 il6 sp1

ECD Proteins

Cynomolgus IL6 Protein, His Tag

SKU:  PME-C100123     Target:  IL6     Tag:  N-10×His tag

Expression Host:  HEK293

Price:10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme c100122b cd79b sp1

ECD Proteins

Biotinylated Cynomolgus CD79B(30-161) Protein, hFc Tag

SKU:  PME-C100122B     Target:  CD79B     Tag:  C-Human Fc Avi tag

Expression Host:  HEK293

Price:10μg $79.00; 50μg $299.00; 100μg $450.00

sp-pme c100122 cd79b sp1

ECD Proteins

Cynomolgus CD79B(30-161) Protein, hFc Tag

SKU:  PME-C100122     Target:  CD79B     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme c100121b cd79b sp1

ECD Proteins

Biotinylated Cynomolgus CD79B(30-161) Protein, His Tag

SKU:  PME-C100121B     Target:  CD79B     Tag:  C-10×His Avi Tag

Expression Host:  HEK293

Price:10μg $110.00 ; 50μg $394.00 ; 100μg $581.00

sp-pme c100121 cd79b sp1

ECD Proteins

Cynomolgus CD79B(30-161) Protein, His Tag

SKU:  PME-C100121     Target:  CD79B     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme c100120 il18 sp1

ECD Proteins

Cynomolgus IL18 Protein, hFc Tag

SKU:  PME-C100120     Target:  IL18     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101992 cd5 sp1

ECD Proteins

Human CD5(269-372) Protein, hFc Tag

SKU:  PME101992     Target:  CD5     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101991 cd5 sp1

ECD Proteins

Human CD5(156-275) Protein, hFc Tag

SKU:  PME101991     Target:  CD5     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101990 cd5 sp1

ECD Proteins

Human CD5(134-158) Protein, hFc Tag

SKU:  PME101990     Target:  CD5     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101989 cd5 sp1

ECD Proteins

Human CD5(25-135) Protein, hFc Tag

SKU:  PME101989     Target:  CD5     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101988b cd300a sp1

ECD Proteins

Biotinylated Human CD300A Protein, His Tag

SKU:  PME101988B     Target:  CD300A     Tag:  C-6×His Avi Tag

Expression Host:  HEK293

Price:10μg $105.00 ; 50μg $363.00 ; 100μg $550.00

sp-pme101988 cd300a sp1

ECD Proteins

Human CD300A Protein, His Tag

SKU:  PME101988     Target:  CD300A     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101987b cd300a sp1

ECD Proteins

Biotinylated Human CD300A(R111Q) Protein, His Tag

SKU:  PME101987B     Target:  CD300A     Tag:  C-6×His Avi Tag

Expression Host:  HEK293

Price:10μg $105.00 ; 50μg $363.00 ; 100μg $550.00

sp-pme101987 cd300a sp1

ECD Proteins

Human CD300A(R111Q) Protein, His Tag

SKU:  PME101987     Target:  CD300A     Tag:  C-6×His tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101986b cd300a sp1

ECD Proteins

Biotinylated Human CD300A(R111Q) Protein, hFc Tag

SKU:  PME101986B     Target:  CD300A     Tag:  C-Human Fc Avi tag

Expression Host:  HEK293

Price:10μg $99.00 ; 50μg $352.00 ; 100μg $528.00

sp-pme101986 cd300a sp1

ECD Proteins

Human CD300A(R111Q) Protein, hFc Tag

SKU:  PME101986     Target:  CD300A     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101985 il2rb il2rg sp1

ECD Proteins

Human IL2RB-knob and IL2RG-Hole Heterodimer Protein, His Tag and Strep tag

SKU:  PME101985     Target:  IL2RB and IL2RG     Tag:  C-6×His tag and C-Strep tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101984 casr sp1

ECD Proteins

Human CASR Protein, His Tag

SKU:  PME101984     Target:  CASR     Tag:  C-10×His tag

Expression Host:  HEK293

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101983 gpr68 sp1

ECD Proteins

Human GPR68 Protein, hFc Tag

SKU:  PME101983     Target:  GPR68     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101982 cxcr3 sp1

ECD Proteins

Human CXCR3-B(1-51) Protein, mFc Tag

SKU:  PME101982     Target:  CXCR3     Tag:  C-Mouse Fc tag

Expression Host:  HEK293

Price:10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101981 trbv9 sp1

ECD Proteins

Human TRBV9 Protein, hFc Tag

SKU:  PME101981     Target:  TRBV9     Tag:  C-Human Fc tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101733b il33 sp1

ECD Proteins

Biotinylated Human IL33(109-270) Protein, His Tag

SKU:  PME101733B     Target:  IL33     Tag:  N-6×His Avi Tag

Expression Host:  HEK293

Price:10μg $105.00 ; 50μg $363.00 ; 100μg $550.00

sp-pme100625b fcrl5 sp1

ECD Proteins

Biotinylated Human FCRL5 Protein, His Tag

SKU:  PME100625B     Target:  FCRL5     Tag:  C-6×His Avi Tag

Expression Host:  HEK293

Price:10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme f100001b il31 sp1

ECD Proteins

Biotinylated Feline IL31 Protein, His Tag

SKU:  PME-F100001B     Target:  IL31     Tag:  C-6×His Avi Tag

Expression Host:  HEK293

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme d100004b il31 sp1

ECD Proteins

Biotinylated Canine IL31 Protein, His Tag

SKU:  PME-D100004B     Target:  IL31     Tag:  C-6×His Avi Tag

Expression Host:  HEK293

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100781b cd19 sp1

ECD Proteins

Biotinylated Human CD19 Protein, mFc Tag

SKU:  PME100781B     Target:  CD19     Tag:  C-Mouse Fc Avi tag

Expression Host:  HEK293

Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme d100006b pd1 sp1

ECD Proteins

Biotinylated Canine PD-1 Protein, mFc Tag

SKU:  PME-D100006B     Target:  PD1     Tag:  C-Mouse Fc Avi tag

Expression Host:  HEK293

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme100840b il7ra sp1

ECD Proteins

Biotinylated Human IL7RA Protein, hFc Tag

SKU:  PME100840B     Target:  IL7RA     Tag:  C-Human Fc Avi tag

Expression Host:  HEK293

Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101558b cd19 sp1

ECD Proteins

Biotinylated Human CD19(M75V,R76S,F85S) Protein, hFc Tag

SKU:  PME101558B     Target:  CD19     Tag:  C-Human Fc Avi tag

Expression Host:  HEK293

Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101051b fgl1 sp1

ECD Proteins

Biotinylated Human FGL1 Protein, hFc Tag

SKU:  PME101051B     Target:  FGL1     Tag:  N-Avi Human Fc tag

Expression Host:  HEK293

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme100025b pd 1 sp1

ECD Proteins

Biotinylated Human PD-1 Protein, mFc-His tag

SKU:  PME100025B     Target:  PD-1     Tag:  C-Mouse Fc and 6×His Avi tag

Expression Host:  HEK293

Price:10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100054b 4 1bb ligand sp1

ECD Proteins

Biotinylated Human 4-1BB Ligand Protein, mFc-His Tag

SKU:  PME100054B     Target:  4-1BB Ligand     Tag:  N-Mouse Fc and C-6×His Avi tag

Expression Host:  HEK293

Price:10μg $85.00; 50μg $330.00 ; 100 μg $500.00