Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101957 crtam sp1

ECD Proteins

Human CRTAM(115-287) Protein, hFc Tag

SKU:  PME101957     Target:  CRTAM     Tag:  C-Human Fc tag

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme101954 ly6g6d sp1

ECD Proteins

Human LY6G6D Protein, hFc Tag

SKU:  PME101954     Target:  LY6G6D     Tag:  N-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101953 snca sp1

ECD Proteins

Human SNCA Protein, hFc Tag

SKU:  PME101953     Target:  SNCA     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101952 gpr88 sp1

ECD Proteins

Human GPR88 Protein, hFc Tag

SKU:  PME101952     Target:  GPR88     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101951 tfrc sp1

ECD Proteins

Human TFRC(121-760) Protein, His Tag

SKU:  PME101951     Target:  TFRC     Tag:  N-10×His tag

Price:10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme101950 sirpα sp1

ECD Proteins

Human SIRPα V8 Protein, His Tag

SKU:  PME101950     Target:  SIRPα     Tag:  C-10×His tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101949 sirpα sp1

ECD Proteins

Human SIRPα V8 Protein, mFc Tag

SKU:  PME101949     Target:  SIRPα     Tag:  C-Mouse Fc tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101948 sirpα sp1

ECD Proteins

Human SIRPα V2 Protein, mFc Tag

SKU:  PME101948     Target:  SIRPα     Tag:  C-Mouse Fc tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101947 sirpα sp1

ECD Proteins

Human SIRPα V2 Protein, His Tag

SKU:  PME101947     Target:  SIRPα     Tag:  C-10×His tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101946 sirpα sp1

ECD Proteins

Human SIRPα V8 Protein, hFc Tag

SKU:  PME101946     Target:  SIRPα     Tag:  C-Human Fc tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101945 sirpα sp1

ECD Proteins

Human SIRPα V2 Protein, hFc Tag

SKU:  PME101945     Target:  SIRPα     Tag:  C-Human Fc tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101944 gabbr2 sp1

ECD Proteins

Human GABBR2 Protein, His Tag

SKU:  PME101944     Target:  GABBR2     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101943 ccrl2 sp1

ECD Proteins

Human CCRL2 Protein, hFc Tag

SKU:  PME101943     Target:  CCRL2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101942 cckbr sp1

ECD Proteins

Human CCKBR Protein, hFc Tag

SKU:  PME101942     Target:  CCKBR     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101941 taar1 sp1

ECD Proteins

Human TAAR1 Protein, hFc Tag

SKU:  PME101941     Target:  TAAR1     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101940 il2rg sp1

ECD Proteins

Human IL2RG Protein, Strep Tag

SKU:  PME101940     Target:  IL2RG     Tag:  C-Strep tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101939 gpr4 sp1

ECD Proteins

Human GPR4 Protein, hFc Tag

SKU:  PME101939     Target:  GPR4     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101938 galr1 sp1

ECD Proteins

Human GALR1 Protein, hFc Tag

SKU:  PME101938     Target:  GALR1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101937 galr2 sp1

ECD Proteins

Human GALR2 Protein, hFc Tag

SKU:  PME101937     Target:  GALR2     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101936 fzd3 sp1

ECD Proteins

Human FZD3 Protein, hFc Tag

SKU:  PME101936     Target:  FZD3     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101935 drd5 sp1

ECD Proteins

Human DRD5 Protein, hFc Tag

SKU:  PME101935     Target:  DRD5     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101934 cysltr1 sp1

ECD Proteins

Human CYSLTR1 Protein, hFc Tag

SKU:  PME101934     Target:  CYSLTR1     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101933 crhr1 sp1

ECD Proteins

Human CRHR1 Protein, hFc Tag

SKU:  PME101933     Target:  CRHR1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

elisa-pme101956b nkg2d elisa1

ECD Proteins

Biotinylated Human NKG2D Protein, hFc Tag

SKU:  PME101956B     Target:  NKG2D     Tag:  N-Avi Human Fc tag

Price:10μg $90.00; 50μg $352.00 ; 100 μg $528.00

elisa-pme101955b pd l1 elisa1

ECD Proteins

Biotinylated Human PD-L1 Protein, hFc Tag

SKU:  PME101955B     Target:  PD-L1     Tag:  C-Human Fc Avi tag

Price:10μg $106.00; 50μg $407.00 ; 100 μg $611.00

elisa-pme100480b pd l1 elisa1

ECD Proteins

Biotinylated Human PD-L1 Protein, His tag

SKU:  PME100480B     Target:  PD-L1     Tag:  C- Avi 6×HisTag

Price:10μg $79.00; 50μg $301.00 ; 100 μg $452.00

elisa-pme100775b tfrc elisa1

ECD Proteins

Biotinylated Human TFRC Protein, His Tag

SKU:  PME100775B     Target:  TFRC     Tag:  N-Avi 6×His Tag

Price:10μg $113.00 ; 50μg $427.00 ; 100 μg $625.00

elisa-pme c100081b tfrc elisa1

ECD Proteins

Biotinylated Cynomolgus TFRC Protein, His Tag

SKU:  PME-C100081B     Target:  TFRC     Tag:  N-Avi 10×His Tag

Price:10μg $87.00 ; 50μg $310.00 ; 100μg $464.00

elisa-pme100801b cdh17 elisa1

ECD Proteins

Biotinylated Human CDH17 Protein, His Tag

SKU:  PME100801B     Target:  CDH17     Tag:  C-6×His Avi Tag

Price:10μg $79.00; 50μg $299.00; 100μg $450.00

elisa-pme101869b bcma elisa1

ECD Proteins

Biotinylated Human BCMA Protein, hFc Tag

SKU:  PME101869B     Target:  BCMA     Tag:  C-Human Fc LPETG tag

Price:10μg $87.00; 50μg $309.00 ; 100μg $464.00

elisa-pme101843b ox40 ligand elisa1

ECD Proteins

Biotinylated Human OX40 Ligand Protein, hFc Tag

SKU:  PME101843B     Target:  OX40 Ligand     Tag:  N-Avi Human Fc tag

Price:10μg $106.00; 50μg $407.00 ; 100 μg $611.00

elisa-pme101717b acvr2b elisa1

ECD Proteins

Biotinylated Human ACVR2B(19-137) Protein, His Tag

SKU:  PME101717B     Target:  ACVR2B     Tag:  C-10×His Avi Tag

Price:10μg $105.00 ; 50μg $363.00 ; 100μg $550.00