Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101873 mmp1 sp1

ECD Proteins

Human MMP1 Protein, His Tag

SKU:  PME101873     Target:  MMP1     Tag:  C-6×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101871 pdgfb sp1

ECD Proteins

Human PDGFB Protein, hFc Tag

SKU:  PME101871     Target:  PDGFB     Tag:  N-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101870 egfr sp1

ECD Proteins

Human EGFR(25-133) Protein, hFc Tag

SKU:  PME101870     Target:  EGFR     Tag:  C-Human Fc tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101868 egfr sp1

ECD Proteins

Human EGFR(446-621) Protein, hFc Tag

SKU:  PME101868     Target:  EGFR     Tag:  C-Human Fc tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101867 egfr sp1

ECD Proteins

Human EGFR(313-445) Protein, hFc Tag

SKU:  PME101867     Target:  EGFR     Tag:  C-Human Fc tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101866 igfbp4 sp1

ECD Proteins

Human IGFBP4 Protein, His Tag

SKU:  PME101866     Target:  IGFBP4     Tag:  C-6×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101865 tfpi2 sp1

ECD Proteins

Human TFPI2 Protein, hFc Tag

SKU:  PME101865     Target:  TFPI2     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101864 sigirr sp1

ECD Proteins

Human SIGIRR Protein, hFc Tag

SKU:  PME101864     Target:  SIGIRR     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101863 epo sp1

ECD Proteins

Human EPO Protein, hFc Tag

SKU:  PME101863     Target:  EPO     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101862 slitrk6 sp1

ECD Proteins

Human SLITRK6 Protein, His Tag

SKU:  PME101862     Target:  SLITRK6     Tag:  C-6×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101861 siglec6 sp1

ECD Proteins

Human SIGLEC6 Protein, His Tag

SKU:  PME101861     Target:  SIGLEC6     Tag:  C-6×His tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101860 htr2b sp1

ECD Proteins

Human HTR2B Protein, hFc Tag

SKU:  PME101860     Target:  HTR2B     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101859 ltb4r sp1

ECD Proteins

Human LTB4R Protein, hFc Tag

SKU:  PME101859     Target:  LTB4R     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101858 lpar5 sp1

ECD Proteins

Human LPAR5 Protein, hFc Tag

SKU:  PME101858     Target:  LPAR5     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101857 lpar3 sp1

ECD Proteins

Human LPAR3 Protein, hFc Tag

SKU:  PME101857     Target:  LPAR3     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101856 lpar2 sp1

ECD Proteins

Human LPAR2 Protein, hFc Tag

SKU:  PME101856     Target:  LPAR2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101853 mc5r sp1

ECD Proteins

Human MC5R Protein, hFc Tag

SKU:  PME101853     Target:  MC5R     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

elisa-pme101851 fcgrt b2m elisa1

ECD Proteins

Human FCGRT and B2M Heterodimer Protein, His Tag and Flag Tag

SKU:  PME101851     Target:  FCGRT and B2M     Tag:  C-6×His tag and C-Flag tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

elisa-pme101850 fcgrt b2m elisa1

ECD Proteins

Human FCGRT and B2M Heterodimer Protein, His Tag and Strep Tag

SKU:  PME101850     Target:  FCGRT and B2M     Tag:  C-6×His tag and C-Strep tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101849 il20ra sp1

ECD Proteins

Human IL20RA Protein, His Tag

SKU:  PME101849     Target:  IL20RA     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101848 muc17 sp1

ECD Proteins

Human MUC17(4131-4390) Protein, His Tag

SKU:  PME101848     Target:  MUC17     Tag:  C-6×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

elisa-pme101844 lag3 elisa1

ECD Proteins

Human LAG3 Protein, mFc Tag

SKU:  PME101844     Target:  LAG3     Tag:  C-Mouse Fc tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

elisa-pme101843 ox40 ligand elisa1

ECD Proteins

Human OX40 Ligand Protein, hFc Tag

SKU:  PME101843     Target:  OX40 Ligand     Tag:  N-Human Fc tag

Price:10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme101842 cdh17 sp1

ECD Proteins

Human CDH17 Protein, mFc Tag

SKU:  PME101842     Target:  CDH17     Tag:  C-Mouse Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

elisa-pme101841 cdh17 elisa1

ECD Proteins

Human CDH17 Protein, hFc Tag

SKU:  PME101841     Target:  CDH17     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

elisa-pme101840 tnfsf15 elisa1

ECD Proteins

Human TNFSF15 Trimer Protein, hFc Tag

SKU:  PME101840     Target:  TNFSF15     Tag:  N-Human Fc tag

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme101839 opn3 sp1

ECD Proteins

Human OPN3 Protein, hFc Tag

SKU:  PME101839     Target:  OPN3     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101838 nmur1 sp1

ECD Proteins

Human NMUR1 Protein, hFc Tag

SKU:  PME101838     Target:  NMUR1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101837 mrgprd sp1

ECD Proteins

Human MRGPRD Protein, hFc Tag

SKU:  PME101837     Target:  MRGPRD     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101836 mchr1 sp1

ECD Proteins

Human MCHR1 Protein, hFc Tag

SKU:  PME101836     Target:  MCHR1     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme100507b cb1 sp1

ECD Proteins

Biotinylated Human CB1 (1-116) Protein, hFc Tag

SKU:  PME100507B     Target:  CB1     Tag:  C-Human Fc Avi tag

Price:10μg $79.00; 50μg $301.00 ; 100 μg $452.00

sp-pme100125b acpp sp1

ECD Proteins

Biotinylated Human ACPP Protein, His Tag

SKU:  PME100125B     Target:  ACPP     Tag:  C-6×His Avi Tag

Price:10μg $105.00; 50μg $385.00 ; 100 μg $580.00