Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

elisa-pme101716b acvr2a elisa1

ECD Proteins

Biotinylated Human ACVR2A(20-135) Protein, His Tag

SKU:  PME101716B     Target:  ACVR2A     Tag:  N-10×His Avi Tag

Price:10μg $99.00 ; 50μg $352.00 ; 100μg $528.00

sp-pme101928 cd3e cd3g sp1

ECD Proteins

Human CD3E-knob and CD3G-Hole Heterodimer Protein, His Tag and Twin Strep tag

SKU:  PME101928     Target:  CD3E and CD3G     Tag:  C-6×His tag and C-Strep tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101927 cd3d cd3e sp1

ECD Proteins

Human CD3D-knob and CD3E-Hole Heterodimer Protein, His Tag and Twin Strep tag

SKU:  PME101927     Target:  CD3D and CD3E     Tag:  C-6×His tag and C-Strep tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

elisa-pme d100012 il31 elisa1

ECD Proteins

Canine IL31 Protein, hFc Tag

SKU:  PME-D100012     Target:  IL31     Tag:  C-Human Fc tag

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

elisa-pme d100011 il31 elisa1

ECD Proteins

Canine IL31 Protein, mFc Tag

SKU:  PME-D100011     Target:  IL31     Tag:  C-Mouse Fc tag

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme c100096 ccr1 sp1

ECD Proteins

Cynomolgus CCR1 Protein, hFc Tag

SKU:  PME-C100096     Target:  CCR1     Tag:  C-Human Fc tag

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme c100094 klrg1 sp1

ECD Proteins

Cynomolgus KLRG1 Protein, hFc Tag

SKU:  PME-C100094     Target:  KLRG1     Tag:  N-Human Fc tag

Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme m100131 cd8a sp1

ECD Proteins

Mouse CD8A Protein, hFc Tag

SKU:  PME-M100131     Target:  CD8A     Tag:  C-Human Fc tag

Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme m100130 ccr1 sp1

ECD Proteins

Mouse CCR1 Protein, hFc Tag

SKU:  PME-M100130     Target:  CCR1     Tag:  C-Human Fc tag

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme m100128 cxcr1 sp1

ECD Proteins

Mouse CXCR1(1-44) Protein, hFc Tag

SKU:  PME-M100128     Target:  CXCR1     Tag:  C-Human Fc tag

Price:10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100127 klrg1 sp1

ECD Proteins

Mouse KLRG1 Protein, hFc Tag

SKU:  PME-M100127     Target:  KLRG1     Tag:  N-Human Fc tag

Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101932 gpr17 sp1

ECD Proteins

Human GPR17 Protein, hFc Tag

SKU:  PME101932     Target:  GPR17     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101931 gpr15 sp1

ECD Proteins

Human GPR15 Protein, hFc Tag

SKU:  PME101931     Target:  GPR15     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101929 or10j5 sp1

ECD Proteins

Human OR10J5 Protein, hFc Tag

SKU:  PME101929     Target:  OR10J5     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101926 il15 il15ra sp1

ECD Proteins

Human IL15RA and IL15 Protein, mFc Tag

SKU:  PME101926     Target:  IL15 and IL15RA     Tag:  C-Mouse Fc tag and tag Free

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101924 fam171a2 sp1

ECD Proteins

Human FAM171A2 Protein, His Tag

SKU:  PME101924     Target:  FAM171A2     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101923 htr4 sp1

ECD Proteins

Human HTR4 Protein, hFc Tag

SKU:  PME101923     Target:  HTR4     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101922 htr1b sp1

ECD Proteins

Human HTR1B Protein, hFc Tag

SKU:  PME101922     Target:  HTR1B     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101921 hrh1 sp1

ECD Proteins

Human HRH1 Protein, hFc Tag

SKU:  PME101921     Target:  HRH1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101920 hcrtr2 sp1

ECD Proteins

Human HCRTR2 Protein, hFc Tag

SKU:  PME101920     Target:  HCRTR2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101919 hcar2 sp1

ECD Proteins

Human HCAR2 Protein, hFc Tag

SKU:  PME101919     Target:  HCAR2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101918 gpr183 sp1

ECD Proteins

Human GPR183 Protein, hFc Tag

SKU:  PME101918     Target:  GPR183     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101917 gpr161 sp1

ECD Proteins

Human GPR161 Protein, hFc Tag

SKU:  PME101917     Target:  GPR161     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101916 gpr143 sp1

ECD Proteins

Human GPR143 Protein, hFc Tag

SKU:  PME101916     Target:  GPR143     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101915 gpr101 sp1

ECD Proteins

Human GPR101 Protein, hFc Tag

SKU:  PME101915     Target:  GPR101     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101914 gpr18 sp1

ECD Proteins

Human GPR18 Protein, hFc Tag

SKU:  PME101914     Target:  GPR18     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101913 il6st sp1

ECD Proteins

Human IL6ST Protein, His Tag

SKU:  PME101913     Target:  IL6ST     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101912 ffar3 sp1

ECD Proteins

Human FFAR3 Protein, hFc Tag

SKU:  PME101912     Target:  FFAR3     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101911 f2rl2 sp1

ECD Proteins

Human F2RL2 Protein, hFc Tag

SKU:  PME101911     Target:  F2RL2     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101910 ednra sp1

ECD Proteins

Human EDNRA Protein, hFc Tag

SKU:  PME101910     Target:  EDNRA     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101909 drd4 sp1

ECD Proteins

Human DRD4 Protein, hFc Tag

SKU:  PME101909     Target:  DRD4     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101908 drd3 sp1

ECD Proteins

Human DRD3 Protein, hFc Tag

SKU:  PME101908     Target:  DRD3     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00