Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101907 drd1 sp1

ECD Proteins

Human DRD1 Protein, hFc Tag

SKU:  PME101907     Target:  DRD1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101906 crhr2 sp1

ECD Proteins

Human CRHR2 Protein, hFc Tag

SKU:  PME101906     Target:  CRHR2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101905 chrm3 sp1

ECD Proteins

Human CHRM3 Protein, hFc Tag

SKU:  PME101905     Target:  CHRM3     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101904 calcr sp1

ECD Proteins

Human CALCR Protein, hFc Tag

SKU:  PME101904     Target:  CALCR     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101903 c3ar1 sp1

ECD Proteins

Human C3AR1 Protein, hFc Tag

SKU:  PME101903     Target:  C3AR1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101901 tacr3 sp1

ECD Proteins

Human TACR3 Protein, hFc Tag

SKU:  PME101901     Target:  TACR3     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101900 rgr sp1

ECD Proteins

Human RGR Protein, hFc Tag

SKU:  PME101900     Target:  RGR     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101899 p2ry12 sp1

ECD Proteins

Human P2RY12 Protein, hFc Tag

SKU:  PME101899     Target:  P2RY12     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101898 or6c70 sp1

ECD Proteins

Human OR6C70 Protein, hFc Tag

SKU:  PME101898     Target:  OR6C70     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101897 or2t12 sp1

ECD Proteins

Human OR2T12 Protein, hFc Tag

SKU:  PME101897     Target:  OR2T12     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101896 or1j4 sp1

ECD Proteins

Human OR1J4 Protein, hFc Tag

SKU:  PME101896     Target:  OR1J4     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101895 ntsr2 sp1

ECD Proteins

Human NTSR2 Protein, hFc Tag

SKU:  PME101895     Target:  NTSR2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101894 htr6 sp1

ECD Proteins

Human HTR6 Protein, hFc Tag

SKU:  PME101894     Target:  HTR6     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101893 htr2a sp1

ECD Proteins

Human HTR2A Protein, hFc Tag

SKU:  PME101893     Target:  HTR2A     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101892 bilf1 sp1

ECD Proteins

EBVB9 BILF1 Protein, hFc Tag

SKU:  PME101892     Target:  BILF1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

elisa-pme101891 il15 il15ra elisa1

ECD Proteins

Human IL15RA and IL15 Protein, hFc Tag (N120D)

SKU:  PME101891     Target:  IL15 and IL15RA     Tag:  C-Human Fc tag and tag Free

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

elisa-pme101890 igf 1r elisa1

ECD Proteins

Human IGF-1R Protein, mFc Tag

SKU:  PME101890     Target:  IGF-1R     Tag:  C-Mouse Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

elisa-pme101889 igf 1r elisa1

ECD Proteins

Human IGF-1R Protein, hFc Tag

SKU:  PME101889     Target:  IGF-1R     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101888 avpr2 sp1

ECD Proteins

Human AVPR2 Protein, hFc Tag

SKU:  PME101888     Target:  AVPR2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101887 adrb2 sp1

ECD Proteins

Human ADRB2 Protein, hFc Tag

SKU:  PME101887     Target:  ADRB2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101886 adra2a sp1

ECD Proteins

Human ADRA2A Protein, hFc Tag

SKU:  PME101886     Target:  ADRA2A     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101885 ackr1 sp1

ECD Proteins

Human ACKR1 Protein, hFc Tag

SKU:  PME101885     Target:  ACKR1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101884 itgb1 itga3 sp1

ECD Proteins

Human ITGB1 and ITGA3 Heterodimer Protein, hFc Tag and His Tag

SKU:  PME101884     Target:  ITGB1 and ITGA3     Tag:  C-Human Fc tag and C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101882 ptgir sp1

ECD Proteins

Human PTGIR Protein, hFc Tag

SKU:  PME101882     Target:  PTGIR     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101881 gpr45 sp1

ECD Proteins

Human GPR45 Protein, hFc Tag

SKU:  PME101881     Target:  GPR45     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101880 lamp1 sp1

ECD Proteins

Human LAMP1 Protein, His Tag

SKU:  PME101880     Target:  LAMP1     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101879 cdcp1 sp1

ECD Proteins

Human CDCP1(30-368) Protein, hFc Tag

SKU:  PME101879     Target:  CDCP1     Tag:  C-Human Fc tag

Price:10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101878 cdcp1 sp1

ECD Proteins

Human CDCP1(30-430) Protein, His Tag

SKU:  PME101878     Target:  CDCP1     Tag:  C-10×His tag

Price:10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101877 cdcp1 sp1

ECD Proteins

Human CDCP1(30-368) Protein, His Tag

SKU:  PME101877     Target:  CDCP1     Tag:  C-10×His tag

Price:10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101876 vwf sp1

ECD Proteins

Human VWF(764-1238) Protein, His Tag

SKU:  PME101876     Target:  VWF     Tag:  C-10×His tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101875 vwf sp1

ECD Proteins

Human VWF(764-1238) Protein, His Tag

SKU:  PME101875     Target:  VWF     Tag:  N-10×His tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101874 kir2dl5a sp1

ECD Proteins

Human KIR2DL5A Protein, His Tag

SKU:  PME101874     Target:  KIR2DL5A     Tag:  C-6×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00