Extracellular Domain (ECD) Protein
Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.
Features and Applications
Features
Used as lmmunogen for
Therapeutic mAbs
Different Animal Model Species and Fragments
Validated in Therapeutic
mAbs Functional Screening
For Immunization
ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.
Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.
Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram).
For Protein Activity
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.
ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.
Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.
Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.
HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.
Hot Druggable Targets
Immune checkpoint proteins
Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.
Hematological cancer targets
Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.
More hematological cancer
Solid tumor targets
Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.
More solid tumor targetsBlog
The List of ECD Proteins
ECD Proteins
SKU: PME100670 Target: CD166 Tag: C-6×His Tag
Expression Host: HEK293
Price: 10μg $72.00; 50μg $272.00; 100μg $409.00
ECD Proteins
SKU: PME100671 Target: RSPO3 Tag: C-Human Fc Tag
Expression Host: HEK293
Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00
ECD Proteins
SKU: PME100649 Target: KRAS Tag: C-6×His Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME100648 Target: NEFL Tag: N-6×His Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME100647 Target: S protein RBD Tag: C-6×His Tag
Expression Host: HEK293
Price: 10μg $72.00; 50μg $272.00; 100μg $409.00
ECD Proteins
SKU: PME100009 Target: SIRPa Tag: C-Human Fc and 6×His Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME100007 Target: FLT3 Tag: C-Human Fc and 6×His Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME100006 Target: CD138 Tag: C-Human Fc and 6×His Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME100005 Target: CD22 Tag: C-Human Fc and 6×His Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME100004 Target: CD38 Tag: C-Human Fc and 6×His Tag
Expression Host: HEK293
Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00
ECD Proteins
SKU: PME100003 Target: CD123 Tag: C-Human Fc and 6×His Tag
Expression Host: HEK293
Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00
ECD Proteins
SKU: PME100002 Target: CS1 Tag: C-Human Fc and 6×His Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME100752 Target: TNFSF15 Tag: N-Human Fc Tag
Expression Host: HEK293
Price: 10μg $72.00; 50μg $272.00; 100μg $409.00
ECD Proteins
SKU: PME100296 Target: IL17RB Tag: C-6×His Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME100669 Target: LGR4 Tag: C-Human Fc Tag
Expression Host: HEK293
Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00
ECD Proteins
SKU: PME100652 Target: TNFRSF25 Tag: C-Human Fc Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME100633 Target: TREM2 Tag: C-Human Fc Tag
Expression Host: HEK293
Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00
ECD Proteins
SKU: PME100586 Target: IL21R Tag: C-Mouse Fc Tag
Expression Host: HEK293
Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00
ECD Proteins
SKU: PME100399 Target: ROR1 Tag: C-6×His Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME101017 Target: TGFBR2 Tag: C-Human Fc Tag
Expression Host: HEK293
Price: 10μg $72.00; 50μg $272.00; 100μg $409.00
ECD Proteins
SKU: PME100947 Target: GAST Tag: C-Human Fc Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME100868 Target: FOLR2 Tag: C-Human Fc Tag
Expression Host: HEK293
Price:10μg $81.00 ; 50μg $170.00 ; 100μg $234.00
ECD Proteins
SKU: PME100828 Target: CD3E Tag: C-Human Fc Tag
Expression Host: HEK293
Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00
ECD Proteins
SKU: PME100801 Target: CDH17 Tag: C-6×His Tag
Expression Host: HEK293
Price: 10μg $72.00; 50μg $272.00; 100μg $409.00
ECD Proteins
SKU: PME100098 Target: IL2RA Tag: C-6×His Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME100054 Target: 4-1BB Ligand Tag: N-Mouse Fc and C-6×His Tag
Expression Host: HEK293
Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00
ECD Proteins
SKU: PME100092 Target: SIGLEC10 Tag: C-6×His Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME100081 Target: NKp30 Tag: C-Human Fc Tag
Expression Host: HEK293
Price:10μg $81.00 ; 50μg $170.00 ; 100μg $234.00
ECD Proteins
SKU: PME100071 Target: CEACAM5 Tag: C-6×His Tag
Expression Host: HEK293
Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00
ECD Proteins
SKU: PME100035 Target: BCMA Tag: C-Mouse Fc Tag
Expression Host: HEK293
Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00
ECD Proteins
SKU: PME100464 Target: CD7 Tag: C-Mouse Fc Tag
Expression Host: HEK293
Price: 10μg $72.00; 50μg $272.00; 100μg $409.00
ECD Proteins
SKU: PME100029 Target: B7-H2 Tag: C-Mouse Fc and 6×His Tag
Expression Host: HEK293
Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00