| Tag | C-Flag&Strep Tag |
|---|---|
| Target | |
| Synonyms | CHNG1; hTSHR-I; LGR3 |
| Description | Human TSHR-Strep full length protein-synthetic nanodisc |
| Delivery | In Stock |
| Uniprot ID | P16473 |
| Expression Host | HEK293 |
| Protein Families | Druggable Genome, GPCR, Transmembrane |
| Protein Pathways | Autoimmune thyroid disease, Neuroactive ligand-receptor interaction |
| Molecular Weight | The human full length TSHR-Strep protein has a MW of 86.8 kDa |
| Formulation & Reconstitution | Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5% – 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions. Do not use solvents with a pH below 6.5 or those containing high concentrations of divalent metal ions (greater than 5 mM) in subsequent experiments. |
| Storage&Shipping | Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature. |
| Background | The protein is a membrane protein and a major controller of thyroid cell metabolism. The encoded protein is a receptor for thyrothropin and thyrostimulin, and its activity is mediated by adenylate cyclase. Defects in this gene are a cause of several types of hyperthyroidism. |
| Usage | Research use only |
| Conjugate | Unconjugated |
Human TSHR-Strep full length protein-synthetic nanodisc
Product Data Negative control Dima FAQ
Images Negative control Dima FAQ
Figure 1. Elisa plates were pre-coated with C-Flag&Strep Tag TSHR-Strep-Nanodisc (0.2μg/per well). Serial diluted anti-Flag monoclonal antibody solutions were added, washed, and incubated with secondary antibody before Elisa reading. From above data, the EC50 for anti-Flag monoclonal antibody binding with TSHR-Strep-nanodisc is 0.676ng/ml.
Figure 2. Human TSHR-Strep-Nanodisc, C-Flag&Strep Tag on SDS-PAGE
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