Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101806 prokr2 sp1

ECD Proteins

Human PROKR2 Protein, hFc Tag

SKU:  PME101806     Target:  PROKR2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101805 ide sp1

ECD Proteins

Human IDE Protein, His Tag

SKU:  PME101805     Target:  IDE     Tag:  C-10×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101804 lrrc4 sp1

ECD Proteins

Human LRRC4 Protein, His Tag

SKU:  PME101804     Target:  LRRC4     Tag:  C-6×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101803 lilra5 sp1

ECD Proteins

Human LILRA5 Protein, His Tag

SKU:  PME101803     Target:  LILRA5     Tag:  C-6×His tag

Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101802 cntn2 sp1

ECD Proteins

Human CNTN2 Protein, His Tag

SKU:  PME101802     Target:  CNTN2     Tag:  C-6×His tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

elisa-pme101830 cd8a elisa1

ECD Proteins

Human CD8A Acidic tail Protein, His Tag

SKU:  PME101830     Target:  CD8A     Tag:  C-6×His tag

Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101677b bcma sp1

ECD Proteins

Biotinylated Human BCMA Protein, hFc Avi Tag

SKU:  PME101677B     Target:  BCMA     Tag:  C-Human Fc Avi tag

Price:10μg $79.00; 50μg $281.00 ; 100μg $422.00

sp-pme m100125 cxcr2 sp1

ECD Proteins

Mouse CXCR2 Protein, hFc Tag

SKU:  PME-M100125     Target:  CXCR2     Tag:  C-Human Fc tag

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme c100088 cxcr2 sp1

ECD Proteins

Cynomolgus CXCR2(1-63) Protein, hFc Tag

SKU:  PME-C100088     Target:  CXCR2     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme c100087 cd94 sp1

ECD Proteins

Cynomolgus CD94 Protein, hFc Tag

SKU:  PME-C100087     Target:  CD94     Tag:  N-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme c100086 nkg2a sp1

ECD Proteins

Cynomolgus NKG2A Protein, hFc Tag

SKU:  PME-C100086     Target:  NKG2A     Tag:  N-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101801 agtr2 sp1

ECD Proteins

Human AGTR2 Protein, hFc Tag

SKU:  PME101801     Target:  AGTR2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101800 adrb3 sp1

ECD Proteins

Human ADRB3 Protein, hFc Tag

SKU:  PME101800     Target:  ADRB3     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101799 adra1a sp1

ECD Proteins

Human ADRA1A Protein, hFc Tag

SKU:  PME101799     Target:  ADRA1A     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101798 ephb4 sp1

ECD Proteins

Human EPHB4 Protein, His Tag

SKU:  PME101798     Target:  EPHB4     Tag:  C-6×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101797 mc3r sp1

ECD Proteins

Human MC3R Protein, hFc Tag

SKU:  PME101797     Target:  MC3R     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101796 mc2r sp1

ECD Proteins

Human MC2R Protein, hFc Tag

SKU:  PME101796     Target:  MC2R     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101795 fcgrt sp1

ECD Proteins

Human FCGRT Protein, His Tag

SKU:  PME101795     Target:  FCGRT     Tag:  C-10×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101794 vegfb sp1

ECD Proteins

Human VEGFB Protein, hFc Tag

SKU:  PME101794     Target:  VEGFB     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101791 fgf21 sp1

ECD Proteins

Human FGF21 Protein, His Tag

SKU:  PME101791     Target:  FGF21     Tag:  C-10×His tag

Price:10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101790 cd19 sp1

ECD Proteins

Human CD19 Protein, His Tag

SKU:  PME101790     Target:  CD19     Tag:  C-10×His tag

Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101789 pd 1 sp1

ECD Proteins

Human PD-1(25-145) Protein, hFc Tag

SKU:  PME101789     Target:  PD-1     Tag:  C-Human Fc tag

Price:10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme101788 htr7 sp1

ECD Proteins

Human HTR7 Protein, hFc Tag

SKU:  PME101788     Target:  HTR7     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101787 kiss1r sp1

ECD Proteins

Human KISS1R Protein, hFc Tag

SKU:  PME101787     Target:  KISS1R     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101786 lilrb1 sp1

ECD Proteins

Human LILRB1 Protein, His Tag

SKU:  PME101786     Target:  LILRB1     Tag:  C-6×His tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101785 lilra6 sp1

ECD Proteins

Human LILRA6 Protein, His Tag

SKU:  PME101785     Target:  LILRA6     Tag:  C-6×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101784 tacr2 sp1

ECD Proteins

Human TACR2 Protein, hFc Tag

SKU:  PME101784     Target:  TACR2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101783 tacr2 sp1

ECD Proteins

Human TACR2 Protein, mFc Tag

SKU:  PME101783     Target:  TACR2     Tag:  C-Mouse Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101782 lgals4 sp1

ECD Proteins

Human LGALS4 Protein, His Tag

SKU:  PME101782     Target:  LGALS4     Tag:  N-6×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101781 fgf9 sp1

ECD Proteins

Human FGF9 Protein, His Tag

SKU:  PME101781     Target:  FGF9     Tag:  N-6×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101780 lilra1 sp1

ECD Proteins

Human LILRA1 Protein, His Tag

SKU:  PME101780     Target:  LILRA1     Tag:  C-6×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101779 adrb1 sp1

ECD Proteins

Human ADRB1 Protein, hFc Tag

SKU:  PME101779     Target:  ADRB1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00