Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100125 acpp sp1

ECD Proteins

Human ACPP Protein, His Tag

SKU:  PME100125     Target:  ACPP     Tag:  C-10×His tag

Price:10μg $96.00; 50μg $350.00 ; 100 μg $528.00

sp-pme c100089 cd19 sp1

ECD Proteins

Cynomolgus CD19 Protein, His Tag

SKU:  PME-C100089     Target:  CD19     Tag:  C-10×His tag

Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme m100126 il4ra sp1

ECD Proteins

Mouse IL4RA Protein, His Tag

SKU:  PME-M100126     Target:  IL4RA     Tag:  C-10×His tag

Price:10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme c100092 c5ar1 sp1

ECD Proteins

Cynomolgus C5AR1 Protein, hFc Tag

SKU:  PME-C100092     Target:  C5AR1     Tag:  C-Human Fc tag

Price:10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme c100091 gpr75 sp1

ECD Proteins

Cynomolgus GPR75 Protein, hFc Tag

SKU:  PME-C100091     Target:  GPR75     Tag:  C-Human Fc tag

Price:10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme c100090 cd38 sp1

ECD Proteins

Cynomolgus CD38 Protein, His Tag

SKU:  PME-C100090     Target:  CD38     Tag:  N-10×His tag

Price:10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101834 b7 h3 sp1

ECD Proteins

Human B7-H3(27-461) Protein, hFc Tag

SKU:  PME101834     Target:  B7-H3     Tag:  C-Human Fc tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101833 oprd1 sp1

ECD Proteins

Human OPRD1 Protein, hFc Tag

SKU:  PME101833     Target:  OPRD1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101832 opn4 sp1

ECD Proteins

Human OPN4 Protein, hFc Tag

SKU:  PME101832     Target:  OPN4     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101831 b7 h3 sp1

ECD Proteins

Human B7-H3(29-245) Protein, hFc Tag

SKU:  PME101831     Target:  B7-H3     Tag:  C-Human Fc tag

Price:10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme101829 itga4 sp1

ECD Proteins

Human ITGA4(1-977) Acidic tail Protein, hFc Tag

SKU:  PME101829     Target:  ITGA4     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101828 avpr1a sp1

ECD Proteins

Human AVPR1A Protein, hFc Tag

SKU:  PME101828     Target:  AVPR1A     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101827 ackr2 sp1

ECD Proteins

Human ACKR2 Protein, hFc Tag

SKU:  PME101827     Target:  ACKR2     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101826 gpr37 sp1

ECD Proteins

Human GPR37 Protein, His Tag

SKU:  PME101826     Target:  GPR37     Tag:  C-10×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101824 il22ra1 sp1

ECD Proteins

Human IL22RA1 Protein, hFc Tag

SKU:  PME101824     Target:  IL22RA1     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101823 npy2r sp1

ECD Proteins

Human NPY2R Protein, hFc Tag

SKU:  PME101823     Target:  NPY2R     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101822 npy1r sp1

ECD Proteins

Human NPY1R Protein, hFc Tag

SKU:  PME101822     Target:  NPY1R     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101821 kl sp1

ECD Proteins

Human KL Protein, His Tag

SKU:  PME101821     Target:  KL     Tag:  C-6×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101820 igfbp3 sp1

ECD Proteins

Human IGFBP3 Protein, His Tag

SKU:  PME101820     Target:  IGFBP3     Tag:  C-6×His tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101819 il27ra sp1

ECD Proteins

Human IL27RA(33-516) Protein, hFc Tag

SKU:  PME101819     Target:  IL27RA     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101818 olfml3 sp1

ECD Proteins

Human OLFML3 Protein, hFc Tag

SKU:  PME101818     Target:  OLFML3     Tag:  N-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101817 dcn sp1

ECD Proteins

Human DCN(31-359) Protein, His Tag

SKU:  PME101817     Target:  DCN     Tag:  C-6×His tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101816 dcn sp1

ECD Proteins

Human DCN(17-359) Protein, His Tag

SKU:  PME101816     Target:  DCN     Tag:  C-6×His tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101815 vipr2 sp1

ECD Proteins

Human VIPR2 Protein, hFc Tag

SKU:  PME101815     Target:  VIPR2     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101814 vipr1 sp1

ECD Proteins

Human VIPR1 Protein, hFc Tag

SKU:  PME101814     Target:  VIPR1     Tag:  C-Human Fc tag

Price:10μg $95.00 ; 50μg $330.00 ; 100μg $500.00

sp-pme101813 tas2r38 sp1

ECD Proteins

Human TAS2R38 Protein, hFc Tag

SKU:  PME101813     Target:  TAS2R38     Tag:  C-Human Fc tag

Price:10μg $100.00 ; 50μg $358.00 ; 100μg $528.00

sp-pme101812 sstr3 sp1

ECD Proteins

Human SSTR3 Protein, hFc Tag

SKU:  PME101812     Target:  SSTR3     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101811 sstr1 sp1

ECD Proteins

Human SSTR1 Protein, hFc Tag

SKU:  PME101811     Target:  SSTR1     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101810 sctr sp1

ECD Proteins

Human SCTR Protein, hFc Tag

SKU:  PME101810     Target:  SCTR     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101809 s1pr5 sp1

ECD Proteins

Human S1PR5 Protein, hFc Tag

SKU:  PME101809     Target:  S1PR5     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101808 ptgfr sp1

ECD Proteins

Human PTGFR Protein, hFc Tag

SKU:  PME101808     Target:  PTGFR     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00

sp-pme101807 ptger3 sp1

ECD Proteins

Human PTGER3 Protein, hFc Tag

SKU:  PME101807     Target:  PTGER3     Tag:  C-Human Fc tag

Price:10μg $90.00 ; 50μg $320.00 ; 100μg $480.00