Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100023 pdl1 sp1

ECD Proteins

Human PD-L1 Protein, mFc-His tag

SKU:  PME100023     Target:  PDL1     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-PME100017 CTLA 4 Fig.1 SDS PAGE 1

ECD Proteins

Human CTLA-4 Protein, mFc-His Tag

SKU:  PME100017     Target:  CTLA-4     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100012 b7 h3 sp1

ECD Proteins

Human B7-H3 Protein, mFc-His Tag

SKU:  PME100012     Target:  B7-H3     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100655 folr2 sp1

ECD Proteins

Human FOLR2 Protein, His Tag

SKU:  PME100655     Target:  FOLR2     Tag:  C-6×His Tag

Price:10μg $81.00 ; 50μg $170.00 ; 100μg $234.00

sp-pme100008 cd47 sp1

ECD Proteins

Human CD47 Protein, mFc-His Tag

SKU:  PME100008     Target:  CD47     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100001 bcma sp1

ECD Proteins

Human BCMA Protein, hFc-His Tag

SKU:  PME100001     Target:  BCMA     Tag:  C-Human Fc and 6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

Placeholder

ECD Proteins

Human AKT1 Protein, His Tag

SKU:  PME101210     Target:  AKT1     Tag:  C-8×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

Placeholder

ECD Proteins

Human CCL16 Protein

SKU:  PME30080     Target:  CCL16     Tag: 

Price: 50 μg $400.00

Placeholder

ECD Proteins

Human IL-29 (C-10His) Protein

SKU:  PME30045     Target:  IL-29     Tag:  C-10×His Tag

Price: 50 μg $257.00

products-pme

ECD Proteins

Human CD11 Protein, hFc Tag

SKU:  PME101097     Target:  CD11     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human LALBA Protein, hFc Tag

SKU:  PME101096     Target:  LALBA     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human FMNL1 Protein, hFc Tag

SKU:  PME101084     Target:  FMNL1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human LILRA5 Protein, hFc Tag

SKU:  PME101082     Target:  LILRA5     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human GPC2 Protein, hFc Tag

SKU:  PME101074     Target:  GPC2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human TIE2 Protein, hFc Tag

SKU:  PME101072     Target:  TIE2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human CD305 Protein, hFc Tag

SKU:  PME101071     Target:  CD305     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human ULBP2 Protein, hFc Tag

SKU:  PME101066     Target:  ULBP2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human PPT Protein, hFc Tag

SKU:  PME101061     Target:  PPT     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human DLK1 Protein, hFc Tag

SKU:  PME101059     Target:  DLK1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human RET Protein, hFc Tag

SKU:  PME101043     Target:  RET     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human TYRO3 Protein, hFc Tag

SKU:  PME101028     Target:  TYRO3     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human TNFRSF10A Protein, hFc Tag

SKU:  PME101026     Target:  TNFRSF10A     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme101016 tgfb3 sp1

ECD Proteins

Human TGFB3 Protein, hFc Tag

SKU:  PME101016     Target:  TGFB3     Tag:  C-Human Fc tag

Price:10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human PRAME Protein, hFc Tag

SKU:  PME100998     Target:  PRAME     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human POTED Protein, hFc Tag

SKU:  PME100997     Target:  POTED     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human PCDH18 Protein, hFc Tag

SKU:  PME100996     Target:  PCDH18     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human PBF Protein, hFc Tag

SKU:  PME100995     Target:  PBF     Tag:  C-Human Fc Tag

This product is no longer available. Click here to view recommended products

products-pme

ECD Proteins

Human Myeloblastin Protein, hFc Tag

SKU:  PME100986     Target:  Myeloblastin     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human MRP1 Protein, hFc Tag

SKU:  PME100982     Target:  MRP1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human LAMP Protein, hFc Tag

SKU:  PME100971     Target:  LAMP     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100969 klk3 sp1

ECD Proteins

Human KLK3 Protein, hFc Tag

SKU:  PME100969     Target:  KLK3     Tag:  C-Human Fc tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

products-pme

ECD Proteins

Human IGFBP2 Protein, hFc Tag

SKU:  PME100963     Target:  IGFBP2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00