Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100487 s rbd sp1

ECD Proteins

SARS-CoV-2 (2019-nCoV) S protein RBD, hFc Tag

SKU:  PME100487     Target:  S protein RBD     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100498 rbd m sp1

ECD Proteins

SARS-CoV-2 Spike-Membrane Recombinant Fusion Protein, His Tag

SKU:  PME100498     Target:  S protein     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100497 rbd sp1

ECD Proteins

SARS-CoV-2 (2019-nCoV) S protein RBD, mFc Tag

SKU:  PME100497     Target:  S protein RBD     Tag:  C-Mouse Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100496 s1 protein ctd sp1

ECD Proteins

SARS-CoV-2 (2019-nCoV) S1 protein CTD, hFc Tag

SKU:  PME100496     Target:  S1 protein CTD     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100493 s1 protein ntd sp1

ECD Proteins

SARS-CoV-2 (2019-nCoV) S1 protein NTD, hFc Tag

SKU:  PME100493     Target:  S1 protein NTD     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100492 s1 protein ntd sp1

ECD Proteins

SARS-CoV-2 (2019-nCoV) S1 protein NTD, mFc Tag

SKU:  PME100492     Target:  S1 protein NTD     Tag:  C-Mouse Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100491 s1 protein ntd sp1

ECD Proteins

SARS-CoV-2 (2019-nCoV) S1 protein NTD, His Tag

SKU:  PME100491     Target:  S1 protein NTD     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100485 cd155 sp1

ECD Proteins

Human CD155 Protein, hFc tag

SKU:  PME100485     Target:  CD155     Tag:  C-Human Fc Tag

Price: 10μg $91.00; 50μg $348.00 ; 100 μg $522.00

sp-pme100484 icos sp1

ECD Proteins

Human ICOS Protein, hFc tag

SKU:  PME100484     Target:  ICOS     Tag:  C-Human Fc Tag

Price: 10μg $91.00; 50μg $348.00 ; 100 μg $522.00

sp-PME100481 CD30 Fig.1 SDS PAGE 1

ECD Proteins

Human CD30 Protein, His tag

SKU:  PME100481     Target:  CD30     Tag:  C-6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100486 cd70 sp1

ECD Proteins

Human CD70 Protein, hFc-His Tag

SKU:  PME100486     Target:  CD70     Tag:  C-Human Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme101103 ccr8 sp1

ECD Proteins

Human CCR8 Protein, mFc Tag

SKU:  PME101103     Target:  CCR8     Tag:  C-Mouse Fc Tag

Price: 10μg $100.00; 50μg $366.00 ; 100 μg $549.00

sp-pme100474 b7 2 sp1

ECD Proteins

Human B7-2 Protein, hFc Tag

SKU:  PME100474     Target:  B7-2     Tag:  C-Human Fc Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100473 b7 1 sp1

ECD Proteins

Human B7-1 Protein, hFc Tag

SKU:  PME100473     Target:  B7-1     Tag:  C-Human Fc Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100477 2b4 sp1

ECD Proteins

Human 2B4 Protein, hFc tag

SKU:  PME100477     Target:  2B4     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100076 tnfa sp1

ECD Proteins

Human TNFa Protein, His Tag

SKU:  PME100076     Target:  TNFA     Tag:  C-6×His Tag

Price: 10μg $91.00; 50μg $348.00 ; 100 μg $522.00

sp-pme100074 il2rb sp1

ECD Proteins

Human IL2RB Protein, His Tag

SKU:  PME100074     Target:  IL2RB     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100073 ace2 sp1

ECD Proteins

Human ACE2 Protein, hFc Tag

SKU:  PME100073     Target:  ACE2     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100470 cd5 sp1

ECD Proteins

Human CD5 Protein, His Tag

SKU:  PME100470     Target:  CD5     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100466 cd200 sp1

ECD Proteins

Human CD200 Protein, His Tag

SKU:  PME100466     Target:  CD200     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100070 axl sp1

ECD Proteins

Human AXL Protein, His Tag

SKU:  PME100070     Target:  AXL     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100069 fcgr3a sp1

ECD Proteins

Human FCGR3A Protein (F176V), His Tag

SKU:  PME100069     Target:  FCGR3A     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100061 cd52 sp1

ECD Proteins

Human CD52 Protein, mFc Tag

SKU:  PME100061     Target:  CD52     Tag:  C-Mouse Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100042 cd70 sp1

ECD Proteins

Human CD70 Protein, mFc-His Tag

SKU:  PME100042     Target:  CD70     Tag:  N-Mouse Fc and C-6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100039 cd33 sp1

ECD Proteins

Human CD33(18-259) Protein, hFc-His Tag

SKU:  PME100039     Target:  CD33     Tag:  C-Human Fc and 6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100059 ntba sp1

ECD Proteins

Human NTB-A Protein, mFc-His Tag

SKU:  PME100059     Target:  NTB-A     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100469 cd34 sp1

ECD Proteins

Human CD34 Protein, His Tag

SKU:  PME100469     Target:  CD34     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100468 il17ra sp1

ECD Proteins

Human IL17RA Protein, His Tag

SKU:  PME100468     Target:  IL17RA     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100465 tnfrsf10b sp1

ECD Proteins

Human TNFRSF10B Protein, mFc Tag

SKU:  PME100465     Target:  TNFRSF10B     Tag:  C-Mouse Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100067 csf1r sp1

ECD Proteins

Human CSF1R Protein, His Tag

SKU:  PME100067     Target:  CSF1R     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100048 btla sp1

ECD Proteins

Human BTLA Protein, mFc-His Tag

SKU:  PME100048     Target:  BTLA     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100068 epcam sp1

ECD Proteins

Human EPCAM Protein, His Tag

SKU:  PME100068     Target:  EPCAM     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00