Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100050 dnam 1 sp1

ECD Proteins

Human DNAM-1 Protein, mFc-His Tag

SKU:  PME100050     Target:  DNAM1     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100045 epha3 sp1

ECD Proteins

Human EPHA3 Protein, mFc-His Tag

SKU:  PME100045     Target:  EPHA3     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100463 tweakr sp1

ECD Proteins

Human TweakR Protein, mFc Tag

SKU:  PME100463     Target:  TweakR     Tag:  C-Mouse Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100462 pd1 sp1

ECD Proteins

Human PD-1 Protein, hFc-His tag

SKU:  PME100462     Target:  PD-1     Tag:  C-Human Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100461 pd 1 sp1

ECD Proteins

Human PD-1 Protein, His tag

SKU:  PME100461     Target:  PD-1     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100460 s rbd sp1

ECD Proteins

SARS-CoV-2 (2019-nCoV) S protein RBD, mFc-His Tag

SKU:  PME100460     Target:  S protein RBD     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100459 nucleocapsid sp1

ECD Proteins

SARS-CoV-2 (2019-nCoV) Nucleocapsid, N-His Tag

SKU:  PME100459     Target:  Nucleocapsid     Tag:  N-His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100034 b7 2 sp1

ECD Proteins

Human B7-2 Protein, mFc-His Tag

SKU:  PME100034     Target:  B7-2     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100033 flt3lg sp1 1

ECD Proteins

Human FLT3 Ligand Protein, mFc-His Tag

SKU:  PME100033     Target:  FLT3LG     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100032 il6 sp1 1

ECD Proteins

Human IL6 Protein, mFc-His Tag

SKU:  PME100032     Target:  IL6     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100031 msln296 sp1 1

ECD Proteins

Human Mesothelin (296-580) Protein, mFc-His Tag

SKU:  PME100031     Target:  Mesothelin     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100030 tim3 sp1 1

ECD Proteins

Human TIM3 Protein, mFc-His Tag

SKU:  PME100030     Target:  TIM3     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100028 cd96 sp1 1

ECD Proteins

Human CD96 Protein, mFc-His tag

SKU:  PME100028     Target:  CD96     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100027 cd155 sp1 1

ECD Proteins

Human CD155 Protein, mFc-His tag

SKU:  PME100027     Target:  CD155     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100026 icos sp1

ECD Proteins

Human ICOS Protein, mFc-His tag

SKU:  PME100026     Target:  ICOS     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $91.00; 50μg $348.00 ; 100 μg $522.00

sp-pme100024 slamf1 sp1

ECD Proteins

Human SLAMF1 Protein, mFc-His tag

SKU:  PME100024     Target:  SLAMF1     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100022 ox40 sp1

ECD Proteins

Human OX40 Protein, hFc-His tag

SKU:  PME100022     Target:  OX40     Tag:  C-Human Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100021 ox40l sp1

ECD Proteins

Human OX40L Protein, mFc-His tag

SKU:  PME100021     Target:  OX40 Ligand     Tag:  N-Mouse Fc and C-6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100025 pd1 sp1

ECD Proteins

Human PD-1 Protein, mFc-His tag

SKU:  PME100025     Target:  PD-1     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100011 4 1bb sp1

ECD Proteins

Human 4-1BB Protein, mFc-His Tag

SKU:  PME100011     Target:  4-1BB     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100020 gitr ligand sp1

ECD Proteins

Human GITR Ligand Protein, mFc-His tag

SKU:  PME100020     Target:  GITR Ligand     Tag:  N-Mouse Fc and C-6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100019 gitr sp1

ECD Proteins

Human GITR Protein, mFc-His tag

SKU:  PME100019     Target:  GITR     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-PME100018 SP GITR 300

ECD Proteins

Human GITR Protein, hFc-His tag

SKU:  PME100018     Target:  GITR     Tag:  C-Human Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100016 cd48 sp1

ECD Proteins

Human CD48 Protein, mFc-His Tag

SKU:  PME100016     Target:  CD48     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $91.00; 50μg $348.00 ; 100 μg $522.00

sp-pme100015 cd40 sp1

ECD Proteins

Human CD40 Protein, mFc-His Tag

SKU:  PME100015     Target:  CD40     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100014 cd28 sp1

ECD Proteins

Human CD28 Protein, mFc-His Tag

SKU:  PME100014     Target:  CD28     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100013 cd27 sp1

ECD Proteins

Human CD27 Protein, mFc-His Tag

SKU:  PME100013     Target:  CD27     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100010 2b4 sp1

ECD Proteins

Human 2B4 Protein, mFc-His Tag

SKU:  PME100010     Target:  2B4     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100036 s rbd sp1

ECD Proteins

SARS-CoV-2 (2019-nCoV) S protein RBD, His Tag

SKU:  PME100036     Target:  S protein RBD     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100072 ace2 sp1

ECD Proteins

Human ACE2 Protein, mFc Tag

SKU:  PME100072     Target:  ACE2     Tag:  C-Mouse Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100667 gfap sp1

ECD Proteins

Human GFAP (68-377) Protein, His Tag

SKU:  PME100667     Target:  GFAP     Tag:  N-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100657 nucleocapsid sp1

ECD Proteins

SARS-CoV-2 (Delta) Nucleocapsid, C-His Tag

SKU:  PME100657     Target:  Nucleocapsid     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00