Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

Blog

Multi-Species Recombinant Proteins: The Secret Weapon in Antibody Drug Discovery

Explore how recombinant proteins derived from human, mouse, monkey, and dog enable more accurate antigen selection, cross-species validation, and improved...

16
Nov
Domain-Specific Protein Fragments in Therapeutic Antibody Discovery

Using multiple recombinant protein fragments from the same target—such as extracellular domains, functional regions, and membrane-proximal segments—has become an essential...

08
Dec

The List of ECD Proteins

sp-pme m100049 cd48 sp1

ECD Proteins

Mouse CD48 Protein, hFc Tag

SKU:  PME-M100049     Target:  CD48     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100048 tim3 sp1

ECD Proteins

Mouse TIM3 Protein, hFc Tag

SKU:  PME-M100048     Target:  TIM3     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100047 b7 h2 sp1

ECD Proteins

Mouse B7-H2 Protein, hFc Tag

SKU:  PME-M100047     Target:  B7-H2     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100046 b7 2 sp1

ECD Proteins

Mouse B7-2 Protein, hFc Tag

SKU:  PME-M100046     Target:  B7-2     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100045 psca sp1

ECD Proteins

Mouse PSCA Protein, hFc Tag

SKU:  PME-M100045     Target:  PSCA     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100044 b7 1 sp1

ECD Proteins

Mouse B7-1 Protein, hFc Tag

SKU:  PME-M100044     Target:  B7-1     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100043 ox40 sp1

ECD Proteins

Mouse OX40 Protein, hFc Tag

SKU:  PME-M100043     Target:  OX40     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100042 cd40 sp1

ECD Proteins

Mouse CD40 Protein, hFc Tag

SKU:  PME-M100042     Target:  CD40     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme m100041 dnam 1 sp1

ECD Proteins

Mouse DNAM-1 Protein, hFc Tag

SKU:  PME-M100041     Target:  DNAM1     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100040 mesothelin sp1

ECD Proteins

Mouse Mesothelin Protein, His Tag

SKU:  PME-M100040     Target:  Mesothelin     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100039 qsox1 sp1

ECD Proteins

Mouse QSOX1 Protein, His Tag

SKU:  PME-M100039     Target:  QSOX1     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme m100038 adam28 sp1

ECD Proteins

Mouse ADAM28 Protein, hFc Tag

SKU:  PME-M100038     Target:  ADAM28     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100773 ifnar1 sp1

ECD Proteins

Human IFNAR1 Protein, hFc Tag

SKU:  PME100773     Target:  IFNAR1     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100772 s protein rbd sp1

ECD Proteins

SARS-CoV-2 (Omicron BA.2) S protein RBD, hFc Tag

SKU:  PME100772     Target:  S protein RBD     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100771 adamts1 sp1

ECD Proteins

Human ADAMTS1 Protein, His Tag

SKU:  PME100771     Target:  ADAMTS1     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100770 tpsab1 sp1

ECD Proteins

Human TPSAB1 Protein, His Tag

SKU:  PME100770     Target:  TPSAB1     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100769 fgf21 sp1

ECD Proteins

Human FGF21 Protein, hFc Tag

SKU:  PME100769     Target:  FGF21     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100768 b7 h7 sp1

ECD Proteins

Human B7-H7 Protein, hFc Tag

SKU:  PME100768     Target:  B7-H7     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100767 cd72 sp1

ECD Proteins

Human CD72 Protein, hFc Tag

SKU:  PME100767     Target:  CD72     Tag:  N-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100766 trop2 sp1

ECD Proteins

Human Trop2 Protein, His Tag

SKU:  PME100766     Target:  Trop2     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100765 timp4 sp1

ECD Proteins

Human TIMP4 Protein, hFc Tag

SKU:  PME100765     Target:  TIMP4     Tag:  C-Human Fc Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme100764 itgax itgb2 sp1

ECD Proteins

Human ITGAX and ITGB2 Heterodimer Protein, His Tag and hFc Tag

SKU:  PME100764     Target:  ITGAX and ITGB2     Tag:  C-6×His Tag and C-Human Fc Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme100763 cldn5 sp1

ECD Proteins

Human CLDN5 Protein, hFc Tag

SKU:  PME100763     Target:  CLDN5     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100762 rnf43 sp1

ECD Proteins

Human RNF43 Protein, mFc Tag

SKU:  PME100762     Target:  RNF43     Tag:  C-Mouse Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100761 rnf43 sp1

ECD Proteins

Human RNF43 Protein, hFc Tag

SKU:  PME100761     Target:  RNF43     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100760 anxa1 sp1

ECD Proteins

Human ANXA1 Protein, His Tag

SKU:  PME100760     Target:  ANXA1     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100759 liv 1 sp1

ECD Proteins

Human LIV-1 Protein, His Tag

SKU:  PME100759     Target:  LIV-1     Tag:  C-6×His Tag

Price: 10μg $137.00 ; 50μg $513.00 ; 100 μg $752.00

sp-pme100753 adam15 sp1

ECD Proteins

Human ADAM15 Protein, hFc Tag

SKU:  PME100753     Target:  ADAM15     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100661 eda sp1

ECD Proteins

Human EDA Protein, hFc Tag

SKU:  PME100661     Target:  EDA     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100037 ncl sp1

ECD Proteins

Mouse NCL Protein, His Tag

SKU:  PME-M100037     Target:  NCL     Tag:  N-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-PME101158 NLRP3 Fig.1 SDS PAGE 1

ECD Proteins

Human NLRP3 Protein, Flag Tag

SKU:  PME101158     Target:  NLRP3     Tag:  C-Flag Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100063 cldn6 sp1

ECD Proteins

Human CLDN6 Protein, mFc Tag

SKU:  PME100063     Target:  CLDN6     Tag:  C-Mouse Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00