Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101124 npr1 sp1

ECD Proteins

Human NPR1 Protein, hFc Tag

SKU:  PME101124     Target:  NPR1     Tag:  C-Human Fc Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101123 bmp6 sp1

ECD Proteins

Human BMP6 Protein, His Tag

SKU:  PME101123     Target:  BMP6     Tag:  C-6×His Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101120 bst2 sp1

ECD Proteins

Human BST2 Protein, hFc Tag

SKU:  PME101120     Target:  BST2     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101119 fam3d sp1

ECD Proteins

Human FAM3D Protein, hFc Tag

SKU:  PME101119     Target:  FAM3D     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101118 f2rl1 sp1

ECD Proteins

Human F2RL1 Protein, hFc Tag

SKU:  PME101118     Target:  F2RL1     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101117 amigo2 sp1

ECD Proteins

Human AMIGO2 Protein, His Tag

SKU:  PME101117     Target:  AMIGO2     Tag:  C-6×His Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101116 scg3 sp1

ECD Proteins

Human SCG3 Protein, His Tag

SKU:  PME101116     Target:  SCG3     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101115 osmr sp1

ECD Proteins

Human OSMR Protein, His Tag

SKU:  PME101115     Target:  OSMR     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101114 hsd17b13 sp1

ECD Proteins

Human HSD17B13 Protein, Flag Tag

SKU:  PME101114     Target:  HSD17B13     Tag:  C-3×Flag Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101112 ccr4 sp1

ECD Proteins

Human CCR4(1-39) Protein, mFc Tag

SKU:  PME101112     Target:  CCR4     Tag:  C-Mouse Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101110 gpr87 sp1

ECD Proteins

Human GPR87 Protein, mFc Tag

SKU:  PME101110     Target:  GPR87     Tag:  C-Mouse Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101109 ccr2 sp1

ECD Proteins

Human CCR2 Protein, mFc Tag

SKU:  PME101109     Target:  CCR2     Tag:  C-Mouse Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101108 gprc5c sp1

ECD Proteins

Human GPRC5C Protein, mFc Tag

SKU:  PME101108     Target:  GPRC5C     Tag:  C-Mouse Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101107 gprc5b sp1

ECD Proteins

Human GPRC5B Protein, mFc Tag

SKU:  PME101107     Target:  GPRC5B     Tag:  C-Mouse Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101106 gprc5a sp1

ECD Proteins

Human GPRC5A Protein, mFc Tag

SKU:  PME101106     Target:  GPRC5A     Tag:  C-Mouse Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101105 ccr6 sp1

ECD Proteins

Human CCR6 Protein, mFc Tag

SKU:  PME101105     Target:  CCR6     Tag:  C-Mouse Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100799 cxcr3 sp1

ECD Proteins

Human CXCR3 Protein, mFc Tag

SKU:  PME100799     Target:  CXCR3     Tag:  C-Mouse Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme101146 s protein rbd sp1

ECD Proteins

SARS-CoV-2 (Omicron BA.5) S protein RBD, hFc Tag

SKU:  PME101146     Target:  S protein RBD     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme m100072 baff sp1

ECD Proteins

Mouse BAFF Protein, hFc Tag

SKU:  PME-M100072     Target:  BAFF     Tag:  N-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme m100071 4 1bb ligand sp1

ECD Proteins

Mouse 4-1BB Ligand Protein, hFc Tag

SKU:  PME-M100071     Target:  4-1BB Ligand     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100070 nkg2d sp1

ECD Proteins

Mouse NKG2D Protein, hFc Tag

SKU:  PME-M100070     Target:  NKG2D     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100069 ror1 sp1

ECD Proteins

Mouse ROR1 Protein, His Tag

SKU:  PME-M100069     Target:  ROR1     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100068 cd40 ligand sp1

ECD Proteins

Mouse CD40 Ligand Protein, hFc Tag

SKU:  PME-M100068     Target:  CD40 Ligand     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100067 ox40 ligand sp1

ECD Proteins

Mouse OX40 Ligand Protein, hFc Tag

SKU:  PME-M100067     Target:  OX40 Ligand     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100066 gitr ligand sp1

ECD Proteins

Mouse GITR Ligand Protein, hFc Tag

SKU:  PME-M100066     Target:  GITR Ligand     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100065 taci sp1

ECD Proteins

Mouse TACI Protein, hFc Tag

SKU:  PME-M100065     Target:  TACI     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100064 cd34 sp1

ECD Proteins

Mouse CD34 Protein, hFc Tag

SKU:  PME-M100064     Target:  CD34     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100063 il2ra sp1

ECD Proteins

Mouse IL2RA Protein, hFc Tag

SKU:  PME-M100063     Target:  IL2RA     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100062 egfr sp1

ECD Proteins

Mouse EGFR Protein, His Tag

SKU:  PME-M100062     Target:  EGFR     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100061 cd22 sp1

ECD Proteins

Mouse CD22 Protein, His Tag

SKU:  PME-M100061     Target:  CD22     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100060 ceacam5 sp1

ECD Proteins

Mouse CEACAM5 Protein, His Tag

SKU:  PME-M100060     Target:  CEACAM5     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100798 slpi sp1

ECD Proteins

Human SLPI Protein, hFc Tag

SKU:  PME100798     Target:  SLPI     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00