Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100758 sparc sp1

ECD Proteins

Human SPARC Protein, hFc Tag

SKU:  PME100758     Target:  SPARC     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100757 gpr55 sp1

ECD Proteins

Human GPR55(1-21) Protein, hFc Tag

SKU:  PME100757     Target:  GPR55     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100756 clec4c sp1

ECD Proteins

Human CLEC4C Protein, hFc Tag

SKU:  PME100756     Target:  CLEC4C     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100755 b7 h3 sp1

ECD Proteins

Human B7-H3 Protein, mFc Tag

SKU:  PME100755     Target:  B7-H3     Tag:  C-Mouse Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100754 mmp9 sp1

ECD Proteins

Human MMP9(20-707) Protein, His Tag

SKU:  PME100754     Target:  MMP9     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100751 cd142 sp1

ECD Proteins

Human CD142 Protein, hFc Tag

SKU:  PME100751     Target:  CD142     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100750 prlr sp1

ECD Proteins

Human PRLR Protein, hFc Tag

SKU:  PME100750     Target:  PRLR     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100749 emcn sp1

ECD Proteins

Human EMCN Protein, hFc Tag

SKU:  PME100749     Target:  EMCN     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100748 il1r2 sp1

ECD Proteins

Human IL1R2 Protein, His Tag

SKU:  PME100748     Target:  IL1R2     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100747 rage sp1

ECD Proteins

Human RAGE Protein, His Tag

SKU:  PME100747     Target:  RAGE     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100746 il1rap sp1

ECD Proteins

Human IL1RAP Protein, His Tag

SKU:  PME100746     Target:  IL1RAP     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100740 clec2d sp1

ECD Proteins

Human CLEC2D Protein, His Tag

SKU:  PME100740     Target:  CLEC2D     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100714 hsd17b10 sp1

ECD Proteins

Human HSD17B10 Protein, S Tag

SKU:  PME100714     Target:  HSD17B10     Tag:  N-S Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100745 lilrb2 sp1

ECD Proteins

Human LILRB2 Protein, His Tag

SKU:  PME100745     Target:  LILRB2     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme d100002 anxa2 sp1

ECD Proteins

Canis lupus familiaris ANXA2 Protein, His Tag

SKU:  PME-D100002     Target:  ANXA2     Tag:  C-6×His Tag

Price:10μg $95.00; 50μg $330.00; 100μg $500.00

sp-pme100744 lif sp1

ECD Proteins

Human LIF Protein, hFc Tag

SKU:  PME100744     Target:  LIF     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100743 cd63 sp1

ECD Proteins

Human CD63 Protein, hFc Tag

SKU:  PME100743     Target:  CD63     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100741 adgre2 sp1

ECD Proteins

Human ADGRE2 Protein, His Tag

SKU:  PME100741     Target:  ADGRE2     Tag:  C-7×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100739 il31 sp1

ECD Proteins

Human IL31 Protein, hFc Tag

SKU:  PME100739     Target:  IL31     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100738 mdk sp1

ECD Proteins

Human MDK Protein, hFc Tag

SKU:  PME100738     Target:  MDK     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100737 ifnar1 sp1

ECD Proteins

Human IFNAR1 Protein, mFc Tag

SKU:  PME100737     Target:  IFNAR1     Tag:  C-Mouse Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100736 ret sp1

ECD Proteins

Human RET Protein, His Tag

SKU:  PME100736     Target:  RET     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100735 muc18 sp1

ECD Proteins

Human MUC18 Protein, His Tag

SKU:  PME100735     Target:  MUC18     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100734 lrig1 sp1

ECD Proteins

Human LRIG1 Protein, His Tag

SKU:  PME100734     Target:  LRIG1     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100733 zp3 sp1

ECD Proteins

Human ZP3 Protein, His Tag

SKU:  PME100733     Target:  ZP3     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100729 tmigd2 sp1

ECD Proteins

Human TMIGD2 Protein, hFc Tag

SKU:  PME100729     Target:  TMIGD2     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100728 prnp sp1

ECD Proteins

Human PRNP(90-230) Protein, hFc Tag

SKU:  PME100728     Target:  PRNP     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100727 prnp sp1

ECD Proteins

Human PRNP Protein, hFc Tag

SKU:  PME100727     Target:  PRNP     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100726 flt1 sp1

ECD Proteins

Human FLT1 Protein, His Tag

SKU:  PME100726     Target:  FLT1     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100725 adam8 sp1

ECD Proteins

Human ADAM8 Protein, His Tag

SKU:  PME100725     Target:  ADAM8     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100724 mesothelin sp1

ECD Proteins

Human Mesothelin(37-286) Protein, His Tag

SKU:  PME100724     Target:  Mesothelin     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100723 tem1 sp1

ECD Proteins

Human TEM1 Protein, His Tag

SKU:  PME100723     Target:  TEM1     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00