Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme101034 xage1a sp1

ECD Proteins

Human XAGE1A Protein, hFc Tag

SKU:  PME101034     Target:  XAGE1A     Tag:  N-Human Fc Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme101152 a35r sp1

ECD Proteins

Monkeypox virus A35R Protein, His Tag

SKU:  PME101152     Target:  A35R     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101151 a29l sp1

ECD Proteins

Monkeypox virus A29L Protein, His Tag

SKU:  PME101151     Target:  A29L     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme m100076 asgr1 sp1

ECD Proteins

Mouse ASGR1 Protein, His Tag

SKU:  PME-M100076     Target:  ASGR1     Tag:  N-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme m100075 bmp6 sp1

ECD Proteins

Mouse BMP6 Protein, His Tag

SKU:  PME-M100075     Target:  BMP6     Tag:  C-6×His Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme c100011 btn3a1 sp1

ECD Proteins

Cynomolgus BTN3A1 Protein, His Tag

SKU:  PME-C100011     Target:  BTN3A1     Tag:  C-6×His Tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-pme101150 ceacam8 sp1

ECD Proteins

Human CEACAM8 Protein, His Tag

SKU:  PME101150     Target:  CEACAM8     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101149 fgfr3 sp1

ECD Proteins

Human FGFR3 Protein, His Tag

SKU:  PME101149     Target:  FGFR3     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101148 pcdh18 sp1

ECD Proteins

Human PCDH18 Protein, His Tag

SKU:  PME101148     Target:  PCDH18     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101147 dlk1 sp1

ECD Proteins

Human DLK1 Protein, His Tag

SKU:  PME101147     Target:  DLK1     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101145 bcam sp1

ECD Proteins

Human BCAM Protein, His Tag

SKU:  PME101145     Target:  BCAM     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101144 cd106 sp1

ECD Proteins

Human CD106 Protein, hFc Tag

SKU:  PME101144     Target:  CD106     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101143 lamp3 sp1

ECD Proteins

Human LAMP3 Protein, His Tag

SKU:  PME101143     Target:  LAMP3     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101142 btn3a2 sp1

ECD Proteins

Human BTN3A2 Protein, His Tag

SKU:  PME101142     Target:  BTN3A2     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101141 asgr1 sp1

ECD Proteins

Human ASGR1 Protein, His Tag

SKU:  PME101141     Target:  ASGR1     Tag:  N-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101140 furin sp1

ECD Proteins

Human FURIN Protein, His Tag

SKU:  PME101140     Target:  FURIN     Tag:  C-6×His Tag

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

fc-PME100559P PE GPRC5D mFc FLOW Fig.1

ECD Proteins

PE-conjugated Human GPRC5D Protein, mFc Tag

SKU:  PME100559P     Target:  GPRC5D     Tag:  C-Mouse Fc Tag

Price: 25test $340.00

sp-pme101125 igfbp2 sp1

ECD Proteins

Human IGFBP2 Protein, His Tag

SKU:  PME101125     Target:  IGFBP2     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101139 btn3a3 sp1

ECD Proteins

Human BTN3A3 Protein, His Tag

SKU:  PME101139     Target:  BTN3A3     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101138 cd94 sp1

ECD Proteins

Human CD94 Protein, hFc Tag

SKU:  PME101138     Target:  CD94     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101137 tspan8 sp1

ECD Proteins

Human TSPAN8 Protein, hFc Tag

SKU:  PME101137     Target:  TSPAN8     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101136 pyy sp1

ECD Proteins

Human PYY Protein, hFc Tag

SKU:  PME101136     Target:  PYY     Tag:  N-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101135 retn sp1

ECD Proteins

Human RETN Protein, hFc Tag

SKU:  PME101135     Target:  RETN     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101134 klk3 sp1

ECD Proteins

Human KLK3 Protein, His Tag

SKU:  PME101134     Target:  KLK3     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101133 il12b sp1

ECD Proteins

Human IL12B Protein, His Tag

SKU:  PME101133     Target:  IL12B     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101111 cdh6 sp1

ECD Proteins

Human CDH6 Protein, His Tag

SKU:  PME101111     Target:  CDH6     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101132 areg sp1

ECD Proteins

Human AREG Protein, hFc Tag

SKU:  PME101132     Target:  AREG     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101131 sema4d sp1

ECD Proteins

Human SEMA4D Protein, His Tag

SKU:  PME101131     Target:  SEMA4D     Tag:  C-6×His Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101129 cd59 sp1

ECD Proteins

Human CD59 Protein, hFc Tag

SKU:  PME101129     Target:  CD59     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101128 il13 sp1

ECD Proteins

Human IL13(35-146) Protein, hFc Tag

SKU:  PME101128     Target:  IL13     Tag:  C-Human Fc Tag

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme101127 ppt1 sp1

ECD Proteins

Human PPT1 Protein, His Tag

SKU:  PME101127     Target:  PPT1     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme101126 il18bp sp1

ECD Proteins

Human IL18BP Protein, hFc Tag

SKU:  PME101126     Target:  IL18BP     Tag:  C-Human Fc Tag

Price: 10μg $100.00 ; 50μg $375.00 ; 100 μg $550.00