Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100785 ifna1 sp1

ECD Proteins

Human IFNA1 Protein, hFc Tag

SKU:  PME100785     Target:  IFNA1     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100784 mcemp1 sp1

ECD Proteins

Human MCEMP1 Protein, hFc Tag

SKU:  PME100784     Target:  MCEMP1     Tag:  N-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100783 agr2 sp1

ECD Proteins

Human AGR2 Protein, hFc Tag

SKU:  PME100783     Target:  AGR2     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100782 cldn2 sp1

ECD Proteins

Human CLDN2 Protein, hFc Tag

SKU:  PME100782     Target:  CLDN2     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100781 cd19 sp1

ECD Proteins

Human CD19 Protein, mFc Tag

SKU:  PME100781     Target:  CD19     Tag:  C-Mouse Fc Tag

Expression Host:  HEK293

Price: 10μg $125.00 ; 50μg $469.00 ; 100 μg $688.00

sp-pme100780 rspo1 sp1

ECD Proteins

Human RSPO1(21-146) Protein, hFc Tag

SKU:  PME100780     Target:  RSPO1     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100779 rspo1 sp1

ECD Proteins

Human RSPO1(21-263) Protein, His Tag

SKU:  PME100779     Target:  RSPO1     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100778 qsox1 sp1

ECD Proteins

Human QSOX1 Protein, His Tag

SKU:  PME100778     Target:  QSOX1     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100777 tgfbr1 sp1

ECD Proteins

Human TGFBR1 Protein, hFc Tag

SKU:  PME100777     Target:  TGFBR1     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100776 cd52 sp1

ECD Proteins

Human CD52 Protein, hFc Tag

SKU:  PME100776     Target:  CD52     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100775 tfrc sp1

ECD Proteins

Human TFRC Protein, His Tag

SKU:  PME100775     Target:  TFRC     Tag:  N-6×His Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme100774 cd106 sp1

ECD Proteins

Human CD106 Protein, His Tag

SKU:  PME100774     Target:  CD106     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme m100059 tweakr sp1

ECD Proteins

Mouse TweakR Protein, hFc Tag

SKU:  PME-M100059     Target:  TweakR     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100058 slamf1 sp1

ECD Proteins

Mouse SLAMF1 Protein, hFc Tag

SKU:  PME-M100058     Target:  SLAMF1     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100057 tnfrsf10b sp1

ECD Proteins

Mouse TNFRSF10B Protein, hFc Tag

SKU:  PME-M100057     Target:  TNFRSF10B     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100056 slamf5 sp1

ECD Proteins

Mouse SLAMF5 Protein, hFc Tag

SKU:  PME-M100056     Target:  SLAMF5     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100055 cb1 sp1

ECD Proteins

Mouse CB1 Protein, hFc Tag

SKU:  PME-M100055     Target:  CB1     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100054 cd200 sp1

ECD Proteins

Mouse CD200 Protein, hFc Tag

SKU:  PME-M100054     Target:  CD200     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100053 tigit sp1

ECD Proteins

Mouse TIGIT Protein, hFc Tag

SKU:  PME-M100053     Target:  TIGIT     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100052 il17ra sp1

ECD Proteins

Mouse IL17RA Protein, His Tag

SKU:  PME-M100052     Target:  IL17RA     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme m100051 csf1r sp1

ECD Proteins

Mouse CSF1R Protein, His Tag

SKU:  PME-M100051     Target:  CSF1R     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100050 sirp伪 sp1

ECD Proteins

Mouse SIRPa Protein, His Tag

SKU:  PME-M100050     Target:  SIRPa     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100049 cd48 sp1

ECD Proteins

Mouse CD48 Protein, hFc Tag

SKU:  PME-M100049     Target:  CD48     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100048 tim3 sp1

ECD Proteins

Mouse TIM3 Protein, hFc Tag

SKU:  PME-M100048     Target:  TIM3     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100047 b7 h2 sp1

ECD Proteins

Mouse B7-H2 Protein, hFc Tag

SKU:  PME-M100047     Target:  B7-H2     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100046 b7 2 sp1

ECD Proteins

Mouse B7-2 Protein, hFc Tag

SKU:  PME-M100046     Target:  B7-2     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100045 psca sp1

ECD Proteins

Mouse PSCA Protein, hFc Tag

SKU:  PME-M100045     Target:  PSCA     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100044 b7 1 sp1

ECD Proteins

Mouse B7-1 Protein, hFc Tag

SKU:  PME-M100044     Target:  B7-1     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100043 ox40 sp1

ECD Proteins

Mouse OX40 Protein, hFc Tag

SKU:  PME-M100043     Target:  OX40     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100042 cd40 sp1

ECD Proteins

Mouse CD40 Protein, hFc Tag

SKU:  PME-M100042     Target:  CD40     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme m100041 dnam 1 sp1

ECD Proteins

Mouse DNAM-1 Protein, hFc Tag

SKU:  PME-M100041     Target:  DNAM1     Tag:  C-Human Fc Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00

sp-pme m100040 mesothelin sp1

ECD Proteins

Mouse Mesothelin Protein, His Tag

SKU:  PME-M100040     Target:  Mesothelin     Tag:  C-6×His Tag

Expression Host:  HEK293

Price: 10μg $103.00 ; 50μg $388.00 ; 100 μg $568.00