Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100703 b4galt1 sp1

ECD Proteins

Human B4GALT1 Protein, hFc Tag

SKU:  PME100703     Target:  B4GALT1     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100699 mmp13 sp1

ECD Proteins

Human MMP13 Protein, hFc Tag

SKU:  PME100699     Target:  MMP13     Tag:  C-Human Fc Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100698 mmp13 sp1

ECD Proteins

Human MMP13 Protein, His Tag

SKU:  PME100698     Target:  MMP13     Tag:  C-6×His Tag

Price: 10μg $120.00 ; 50μg $450.00 ; 100 μg $660.00

sp-pme100696 igfbp7 sp1

ECD Proteins

Human IGFBP7 Protein, hFc Tag

SKU:  PME100696     Target:  IGFBP7     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme102006 mbp sp1

ECD Proteins

Human MBP Protein, hFc Tag

SKU:  PME102006     Target:  MBP     Tag:  N-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme c100004 ncl sp1

ECD Proteins

Rhesus macaque NCL Protein, His Tag

SKU:  PME-C100004     Target:  NCL     Tag:  N-6×His Tag

Price: 10μg $95.00 ; 50μg $330.00 ; 100 μg $500.00

sp-pme m100027 mfge8 sp1

ECD Proteins

Mouse MFGE8 Protein, His Tag

SKU:  PME-M100027     Target:  MFGE8     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100814 igf 1r sp1

ECD Proteins

Human IGF-1R Protein, His Tag

SKU:  PME100814     Target:  IGF-1R     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100693 cd33 sp1

ECD Proteins

Human CD33(140-259) Protein, hFc Tag

SKU:  PME100693     Target:  CD33     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100692 cdh1 sp1

ECD Proteins

Human CDH1 Protein, mFc Tag

SKU:  PME100692     Target:  CDH1     Tag:  C-Mouse Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100694 pglyrp1 sp1

ECD Proteins

Human PGLYRP1 Protein, hFc Tag

SKU:  PME100694     Target:  PGLYRP1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme m100026 vwf sp1

ECD Proteins

Mouse VWF (23-2813) Protein, His Tag

SKU:  PME-M100026     Target:  VWF     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme m100025 vwf sp1

ECD Proteins

Mouse VWF (764-2813) Protein, His Tag

SKU:  PME-M100025     Target:  VWF     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme m100024 vwf sp1

ECD Proteins

Mouse VWF (23-763) Protein, His Tag

SKU:  PME-M100024     Target:  VWF     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100690 vwf sp1

ECD Proteins

Human VWF (23-2813) Protein, His Tag

SKU:  PME100690     Target:  VWF     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100689 cd93 sp1

ECD Proteins

Human CD93 Protein, hFc Tag

SKU:  PME100689     Target:  CD93     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $450.00

sp-pme100688 nucleocapsid sp1

ECD Proteins

SARS-CoV-2 (Omicron) Nucleocapsid, C-His Tag

SKU:  PME100688     Target:  Nucleocapsid     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme d100001 il18bp sp1

ECD Proteins

Canis lupus familiaris IL18BP Protein, hFc Tag

SKU:  PME-D100001     Target:  IL18BP     Tag:  C-Human Fc Tag

Price: 10μg $100.00 ; 50μg $375.00 ; 100 μg $550.00

sp-pme c100003 il9 sp1

ECD Proteins

Cynomolgus IL9 Protein, hFc Tag

SKU:  PME-C100003     Target:  IL9     Tag:  C-Human Fc Tag

Price:10μg $120.00; 50μg $380.00; 100μg $570.00

sp-pme c100002 cxcl10 sp1

ECD Proteins

Cynomolgus CXCL10 Protein, hFc Tag

SKU:  PME-C100002     Target:  CXCL10     Tag:  C-Human Fc Tag

Price: 10μg $100.00 ; 50μg $375.00 ; 100 μg $550.00

sp-pme c100001 il18bp sp1

ECD Proteins

Cynomolgus IL18BP Protein, hFc Tag

SKU:  PME-C100001     Target:  IL18BP     Tag:  C-Human Fc Tag

Price: 10μg $100.00 ; 50μg $375.00 ; 100 μg $550.00

sp-pme m100023 rnase4 sp1

ECD Proteins

Mouse RNASE4 Protein, hFc Tag

SKU:  PME-M100023     Target:  RNASE4     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme m100022 lama4 sp1

ECD Proteins

Mouse LAMA4 Protein, His Tag

SKU:  PME-M100022     Target:  LAMA4     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme m100021 tenm4 sp1

ECD Proteins

Mouse TENM4 Protein, His Tag

SKU:  PME-M100021     Target:  TENM4     Tag:  N-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme m100020 hbegf sp1

ECD Proteins

Mouse HBEGF (24-160) Protein, His Tag

SKU:  PME-M100020     Target:  HBEGF     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme m100017 ifnb1 sp1

ECD Proteins

Mouse IFNB1 Protein, His Tag

SKU:  PME-M100017     Target:  IFNB1     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme m100016 gitr sp1

ECD Proteins

Mouse GITR Protein, hFc Tag

SKU:  PME-M100016     Target:  GITR     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme m100015 cd33 sp1

ECD Proteins

Mouse CD33 Protein, hFc Tag

SKU:  PME-M100015     Target:  CD33     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme m100014 cd138 sp1

ECD Proteins

Mouse CD138 Protein, hFc Tag

SKU:  PME-M100014     Target:  CD138     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100686 hbegf sp1

ECD Proteins

Human HBEGF Protein, hFc Tag

SKU:  PME100686     Target:  HBEGF     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-PME100685 TENM4 His PAGE 300

ECD Proteins

Human TENM4 Protein, His Tag

SKU:  PME100685     Target:  TENM4     Tag:  N-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100732 lipg sp1

ECD Proteins

Human LIPG Protein, His Tag

SKU:  PME100732     Target:  LIPG     Tag:  C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00