Extracellular Domain (ECD) Protein

Extracellular domain (ECD) proteins are regions of membrane proteins located outside the cell membrane and are essential for signal recognition, ligand binding, and cell–cell communication. DIMA BIOTECH focuses on ECD proteins from single-pass membrane proteins and has successfully expressed 1,600+ high-purity ECDs covering 900+ targets, including GPCRs, immune checkpoints, tumor antigens, and metabolic receptors. 500+ targets are supported by matched flow cytometry antibodies. All ECD proteins are validated by SDS-PAGE for purity and ELISA for activity, enabling antibody screening, functional studies, and structural research in membrane protein drug development.

the picture of extra-cellular domain (ECD)

Features and Applications

Features

Expressed in Mammalian Cell with Native folding and authentic PTMs 

Used as lmmunogen for
Therapeutic mAbs

Different Animal Model Species and Fragments

Validated in Therapeutic
mAbs Functional Screening

For Immunization

The serum titer of LAG3 protein as immunogen

ELISA results show high OD values of anti-LAG3 immune serum after LAG3 protein (PME100512) immunization across serial dilutions, indicating successful generation of high-titer antibodies.

FACS positive clones were obtained per rabbit after LAG3 protein immunization

Approximately 630+ ELISA-positive clones were obtained per rabbit after immunization, of which 260+ were further confirmed as FACS-positive functional clones.

Flow cytometry analysis with Anti-LAG3 (DM160) on HEK293 cells transfected with human LAG3 (Blue histogram) or HEK293 transfected with irrelevant protein (Red histogram). 

For Protein Activity

PME100002-CS1-hFc-His-ELISA-Fig2

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human CS1 protein (PME100002) can bind Anti-CS1 Neutralizing antibody BME100002 in a linear range of 7.81-210.7 ng/ml.

PME100001-BCMA-hFc-His-ELISA

ELISA plate pre-coated by 2 μg/ml (100 μl/well) Human BCMA protein (PME100001) can bind Anti-BCMA Neutralizing antibody (BME100016) in a linear range of 3.71-22.29 ng/ml.

MUC1 SPR Affinity Curve

Loaded Anti-MUC1 mAb (BME100249) on Pro-A Biosensor, can bind Human MUC1 Protein (PME100106) with an affinity constant of 0.432nM.

ACVR2B SPR Affinity Curve

Loaded Anti-ACVR2B mAb (BME100228) on Pro-A Biosensor binds Human ACVR2B Protein (PME101717) with an afinity constant of 0.169nM.

HEK293 cell line transfected with irrelevant protein (B) and human B7-2 (A) were surface stained with Human CD28 protein (PME100014) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

PME100019-GITR-mFc-His-FC-Fig2

HEK293 cell line transfected with irrelevant protein (B) and human GITR Ligand (A) were surface stained with Human GITR protein (PME100019) 1μg/ml followed by Alexa 488-conjugated anti-mouse IgG secondary antibody.

Click to view activity and stability testing data

Hot Druggable Targets

Immune checkpoint proteins

Immune checkpoint proteins (such as PD-1, CTLA-4, and PD-L1) are molecules that regulate immune responses, often by suppressing immune activity to maintain self-tolerance and prevent autoimmunity.

More immune checkpoints

Hematological cancer targets

Hematological cancer targets (such as CD19, CD20, and CD38) are specific molecules involved in blood cancers like leukemia, lymphoma, and myeloma, which are used for targeted therapies such as monoclonal antibodies.

More hematological cancer

Solid tumor targets

Solid tumor targets (such as HER2, EGFR, and VEGF) are specific targets found on the surface of solid tumor cells, such as in breast, lung, and colorectal cancers, which are targeted by therapies like mAbs and small molecules.

More solid tumor targets

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The List of ECD Proteins

sp-pme100038 cd19 sp1

ECD Proteins

Human CD19 Protein, hFc-His tag

SKU:  PME100038     Target:  CD19     Tag:  C-Human Fc and 6×His Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100506 tigit sp1

ECD Proteins

Human TIGIT Protein, His Tag

SKU:  PME100506     Target:  TIGIT     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100505 tigit sp1

ECD Proteins

Human TIGIT Protein, mFc Tag

SKU:  PME100505     Target:  TIGIT     Tag:  C-Mouse Fc Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100107 tigit sp1

ECD Proteins

Human TIGIT Protein, hFc Tag

SKU:  PME100107     Target:  TIGIT     Tag:  C-Human Fc Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme m100035 stab1 sp1

ECD Proteins

Mouse STAB1 Protein, hFc Tag

SKU:  PME-M100035     Target:  STAB1     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100041 cd30 ligand sp1

ECD Proteins

Human CD30 Ligand Protein, mFc-His Tag

SKU:  PME100041     Target:  CD30 Ligand     Tag:  N-Mouse Fc and C-6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100800 cd3d sp1

ECD Proteins

Human CD3D Protein, His Tag

SKU:  PME100800     Target:  CD3D     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $450.00

sp-pme100504 nucleocapsid sp1

ECD Proteins

SARS-CoV-2 (2019-nCoV) Nucleocapsid, C-His Tag

SKU:  PME100504     Target:  Nucleocapsid     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100091 cd37 sp1

ECD Proteins

Human CD37 Protein, hFc Tag

SKU:  PME100091     Target:  CD37     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100088 her3 sp1

ECD Proteins

Human HER3 Protein, His Tag

SKU:  PME100088     Target:  HER3     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100085 il5 sp1

ECD Proteins

Human IL5 Protein, His Tag

SKU:  PME100085     Target:  IL5     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100503 baff r sp1

ECD Proteins

Human BAFF-R Protein, hFc Tag

SKU:  PME100503     Target:  BAFF-R     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100471 cd162 sp1

ECD Proteins

Human CD162 Protein, His Tag

SKU:  PME100471     Target:  SELPLG     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100044 baff r sp1

ECD Proteins

Human BAFF-R Protein, mFc Tag

SKU:  PME100044     Target:  BAFF-R     Tag:  C-Mouse Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100043 baff sp1

ECD Proteins

Human BAFF Protein, hFc Tag

SKU:  PME100043     Target:  BAFF     Tag:  N-Human Fc Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100502 4 1bb sp1

ECD Proteins

Human 4-1BB Protein, His tag

SKU:  PME100502     Target:  4-1BB     Tag:  C-6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100084 psca sp1

ECD Proteins

Human PSCA Protein, hFc Tag

SKU:  PME100084     Target:  PSCA     Tag:  C-Human Fc Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100083 cd114 sp1

ECD Proteins

Human CD114 Protein, His Tag

SKU:  PME100083     Target:  CD114     Tag:  C-6×His Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100079 nkg2d sp1

ECD Proteins

Human NKG2D Protein, mFc Tag

SKU:  PME100079     Target:  NKG2D     Tag:  N-Mouse Fc tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100499 igg1 fc sp1

ECD Proteins

Human IgG1-Fc Protein

SKU:  PME100499     Target:  IgG1-Fc     Tag:  N/A

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00

sp-pme100501 trop2 sp1

ECD Proteins

Human Trop2 Protein, mFc-His Tag

SKU:  PME100501     Target:  Trop2     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100476 cd48 sp1

ECD Proteins

Human CD48 Protein, hFc Tag

SKU:  PME100476     Target:  CD48     Tag:  C-Human Fc Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100093 il5ra sp1

ECD Proteins

Human IL5RA Protein, His Tag

SKU:  PME100093     Target:  IL5RA     Tag:  C-6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100056 btn3a1 sp1

ECD Proteins

Human BTN3A1 Protein, mFc-His Tag

SKU:  PME100056     Target:  BTN3A1     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100480 pd l1 sp1

ECD Proteins

Human PD-L1 Protein, His tag

SKU:  PME100480     Target:  PDL1     Tag:  C-6×His Tag

Price: 10μg $72.00; 50μg $274.00 ; 100 μg $411.00

sp-pme100479 ctla 4 sp1

ECD Proteins

Human CTLA-4 Protein, hFc tag

SKU:  PME100479     Target:  CTLA-4     Tag:  C-Human Fc Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100478 gitr ligand sp1

ECD Proteins

Human GITR Ligand Protein, His tag

SKU:  PME100478     Target:  GITR Ligand     Tag:  C-6×His Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100475 cd27 sp1

ECD Proteins

Human CD27 Protein, hFc Tag

SKU:  PME100475     Target:  CD27     Tag:  C-Human Fc Tag

Price: 10μg $96.00; 50μg $370.00 ; 100 μg $555.00

sp-pme100402 siglec15 sp1

ECD Proteins

Human SIGLEC15 Protein, mFc-His Tag

SKU:  PME100402     Target:  SIGLEC15     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $82.00; 50μg $320.00 ; 100 μg $480.00

sp-pme100087 selp sp1

ECD Proteins

Human SELP Protein, hFc Tag

SKU:  PME100087     Target:  SELP     Tag:  C-Human Fc Tag

Price: 10μg $78.00; 50μg $310.00 ; 100 μg $460.00

sp-pme100062 icam 1 sp1

ECD Proteins

Human ICAM-1 Protein, mFc Tag

SKU:  PME100062     Target:  ICAM-1     Tag:  C-Mouse Fc Tag

Price: 10μg $85.00; 50μg $330.00 ; 100 μg $500.00

sp-pme100058 slamf5 sp1

ECD Proteins

Human SLAMF5 Protein, mFc-His Tag

SKU:  PME100058     Target:  SLAMF5     Tag:  C-Mouse Fc and 6×His Tag

Price: 10μg $72.00; 50μg $272.00; 100μg $409.00